Abstract
The labeling of RNA in young and adult rat brain has been studied by measuring in vitro (tissue slices incubation) the incorporation of labeled uridine into RNA of total tissue and of the various subcellular fractions purified from cerebral hemispheres of 1- and 10-month-old rats. Gel electrophoretic analysis of the newly synthesized nuclear and microsomal RNA was also accomplished. An active metabolism of RNA in adult animals was found; moreover, distinct differences in ribosomal RNA processing in cerebral hemispheres of 1- and 10-month-old rats, with a more rapid processing in the brain of adult animals, were obtained.
Similar content being viewed by others
References
Mandel, P., Wintzerith, M. 1980. Free nucleotides and nucleic acids during brain development. Pages 241–282,in Kumar, S. (ed), Biochemistry of Brain, Pergamon Press, London.
Mac Donnell, P., Huff, K., Grouse, L., andGuroff, G. 1980. Brain nucleic acids. Pages 211–240,in Kumar, S. (ed.), Biochemistry of brain, Pergamon Press, London.
Giuffrida, A. M., Serra, I., Fiore, L., Vanella, A., andCozzolino, A. 1974. Biosynthesis of nucleic acids in different regions of rat brain during postnatal development. Ital J. Biochem. 23:87–95.
Giuffrida, A. M., Cox, D., andMathias, A. P. 1975. RNA polymerase activity in various classes of nuclei from different regions of rat brain during postnatal development. J. Neurochem. 24:749–755.
Giuffrida, A. M., Cambria, A., Serra, I., andAvitabile, M. 1975. Nucleolar and nucleoplasmic RNA polymerase activity in different regions of rat brain during postnatal development. Ital. J. Biochem. 24:288–307.
Berthold, W., andLim, L. 1976. The metabolism of high-molecular-weight ribonucleic acid, including polyadenylated species, in the developing rat brain. Biochem. J. 154:517–527.
Berthold, W., andLim, L. 1976. Nucleo-cytoplasmic relationship of high-molecular weight ribonucleic acid, including polyadenylated species in the developing rat brain. Biochem. J. 154:529–539.
Takahashi, Y., Araki, K., andSuzuki, Y. 1971. Metabolic pattern of RNA in rat brain nucleus shown by gel electrophoresis. Brain Res. 32:179–188.
Giuffrida, A. M., Hamberger, A., andSerra, I. 1976. Biosynthesis of DNA and RNA in neuronal and glial cells from various regions of developing rat brain. J. Neurosci. Res. 2:203–215.
Giuffrida, A. M., Gadaleta, M. N., Serra, I., Renis, M., Geremia, E., Del Prete G., andSaccone, C. 1979. Mitochondrial DNA, RNA and protein synthesis in different regions of developing rat brain. Neurochem. Res. 4:37–52.
Gadaleta, M. N., Giuffrida, A. M., Renis, M., Serra, I., Del Prete, G., Geremia, E., andSaccone, C. 1979. Macromolecular synthesis in mitochondria isolated from different regions of developing rat brain. Neurochem. Res. 4:25–35.
Serra, I., Renis, M., Geremia, E., Grillo, G., Gadaleta, M. N., andGiuffrida, A. M. 1979. Mitochondrial macromolecular biosynthesis in different brain regions of young and adult rats. Bull. Mol. Biol. Med. 4:251–265.
Hall, C., andLim, L. 1978. The metabolism of high molecular weight ribonucleic acid in hypothalamic and cortical regions of developing female rat brain. Biochem. J. 176:511–521.
Mc Ilwain, H., andBuddle, H. L. 1953. Techniques in tissue metabolism. I. A Mechanical chopper. Biochem. J. 53:412–420.
Austoker, J., Cox, D., andMathias, A. P. 1972. Fractionation of nuclei from brain by zonal centrifugation and a study of the ribonucleic acid polymerase activity in the various classes of nuclei. Biochem. J. 129:1139–1155.
Goldberg, M. A. 1971. Protein synthesis in isolated rat brain mitochondria and nerve endings. Brain Res. 27:319–328.
Schmidt, G., andTannhauser, S. J. 1945. A method for the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animals tissue. J. Biol. Chem. 161:83–89.
Wannemacher, R. W. Jr., Banks, W. L. Jr., andWunner, W. H. 1965. Use a single tissue extract to determine cellular protein and nucleic acid concentrations and rate of amino acid incorporation. Anal. Biochem. 11:320–326.
Plagemann, P. G. W. 1971. Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. I. Kinetics of incorporation of nucleosides into nucleotide pools and pool sizes during growth cycle. J. Cell Physiol. 77:213–240.
Cupello, A., andHyden, H. 1975. Fractionation of RNA from brain synaptosomes and cytoplasmic subcellular fractions. J. Neurochem. 25:399–406.
Avitabile, M., Serra, I., Mathias, A. P., andGiuffrida, A. M. 1981. Effect of undernutrition on RNA synthesis in various regions of developing rat brain. Bull. Mol. Biol. Med. 6:32–43.
Loening, V. E. 1967. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide gel electrophoresis. Biochem. J. 102:251–257.
Gross, N. J., Godfrey, S. G., andRabinowitz, M. 1969. Apparent turnover of mitochondrial deoxyribonucleic acid and mitochondrial phospholipids in the tissue of the rat. J. Biol. Chem. 244:1552–1562.
Saccone, C., andGadaleta, M. N., 1982. Biogenesis and differentiation of animal mitochondria during development. Pages 119–126,in Giuffrida Stella, A. M., Gombos, G., Benzi, G., Bachelard, H. (eds.), Basic and Clinical aspects of Molecular Neurobiology, International Menarini Foundation Publishers, Milano, Italy.
Mandel, P., andEdel-Hart, S. 1966. Free nucleotides in the rat brain during postnatal development. J. Neurochem. 13:591–595.
Judes, C., andJacob, M. 1973. Differentiation of chick embryo cerebral hemispheres. II. Incorporation of3H-uridine into 29 S, 18 S, 5 S and 4 S RNA. Brain Res. 51:253–267.
Cupello, A., Rapallino, M. V., andHyden, H. 1981. Yield of total and poly(A) associated RNA from the cerebral cortex of young and aged rats. IRCS Medical Science 9:976.
Colman, P. D., Kaplan, B. B., Osterburg, H. H., andFinch, C. E. 1980. Brain poly(A) RNA during aging: Stability of yield and sequence complexity in two rat strains. J. Neurochem. 34:335–345.
Hahn, W. E., andChandhari, N. 1981. Late expression of genes represented by poly(A−) mRNA in developing brain. Am. Soc. Neurochem. Abstr. 12:71.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Serra, I., Cupello, A., Gadaleta, M.N. et al. Labeling of RNA in young and adult rat brain: Evidence for different RNA processing. Neurochem Res 8, 433–447 (1983). https://doi.org/10.1007/BF00965100
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00965100