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Subcellular distribution of diacylglycerol lipase in rat and mouse brain

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Abstract

Rat Brain has a lipase which hydrolyzes diacylglycerol at an optimal pH of 4.8 (1). The subcellular distribution of this acid diacylglycerol lipase was studied in brain tissue of rats and mice; in the latter case neurological mutants and their normal controls were used. Several other acidic hydrolases were employed as lysosomal markers. In mouse brain, the specific activity which is about 50–100 times lower than in rat brain, was greatest in the lysosomal fraction. In contrast, no enrichment of DG-lipase was observed in any subcellular fraction of the active enzyme of rat brain. Activities were about equally distributed in the microsomal, myelin-synaptosomal and lysosomal fractions.

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Abbreviations

ARS:

Arylsulfatase

DG:

Diacylglycerol

SPMase:

Sphingomyelinase

HEX:

Hexosaminidase B

Pase:

Acid phosphatase

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Authors and Affiliations

  1. Laboratory of Neurochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel

    A. Rousseau & S. Gatt

  2. Laboratory of Neurochemistry Inserm U-134, Hopital de la Salpetriere, Paris, France

    G. Dubois

Authors
  1. A. Rousseau
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  2. G. Dubois
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  3. S. Gatt
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Rousseau, A., Dubois, G. & Gatt, S. Subcellular distribution of diacylglycerol lipase in rat and mouse brain. Neurochem Res 8, 417–422 (1983). https://doi.org/10.1007/BF00965098

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  • DOI: https://doi.org/10.1007/BF00965098

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