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Neurochemical Research

, Volume 7, Issue 12, pp 1453–1463 | Cite as

Diffusion of intracerebrally injected [1-14C]arachidonic acid and [2-3H]Glycerol in the mouse brain

Effects of ischemia and electroconvulsive shock
  • Maria F. Pediconi
  • Elena B. Rodriguez de Turco
  • Nicolas G. Bazan
Original Articles

Abstract

[2-3H]Glycerol and [1-14C]arachidonic acid were injected into the region of the frontal horn of the left ventricle of mice and were distributed rapidly throughout the brain. After 10 sec, most of the radioactive fatty acid was found in the hemisphere near the injection site; after 10 min, it was recovered in similar proportions in the cerebellum and brain stem. [2-3H]Glycerol showed a heterogeneous distribution, with most of the label remaining in the left hemisphere even after 10 min. On a fresh weight basis, cerebrum, cerebellum, and brain stem were found to contain similar amounts of labeled glycerol. However, the amount of [1-14C]arachidonate in cerebrum was only 50% of that recovered from cerebellum or brain stem. Brain ischemia or a single electroconvulsive shock reduced the spread of the label, producing an accumulation of radioactivity in the injected hemisphere, except for an increase in [2-3H]glycerol in the brain stem during ischemia. Despite the significant decrease in available precursor in the cerebellum and brain stem after electroshock, the amount of label incorporated into lipids was not altered in these areas and only slightly diminished in the cerebrum.

Keywords

Glycerol Ischemia Left Ventricle Arachidonic Acid Mouse Brain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • Maria F. Pediconi
    • 1
  • Elena B. Rodriguez de Turco
    • 1
  • Nicolas G. Bazan
    • 1
  1. 1.Instituto de Investigaciones BioquimicasUniversidad Nacional del Sur-Consejo Nacional de Investigaciones Cientificas y TechnicasBahia BlancaArgentina

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