Abstract
Present study demonstrates differences between the labeling patterns of rat cerebral cortex and cerebellum. Although the initial rates of synthesis and methylation of tRNA from these two regions of brain are different, their final degree of methylation is the same in both the cases. Differences in the rates of incorporation of radioactivities in both the tissues can be explained by the differences in the rates of equilibrium of the labeled precursors with the pools. Increased incorporation of labeled uridine into cerebral cortical tRNA following dibutyryl cAMP treatment probably resulted from higher uptake of the labeled precursor due to permeability changes.
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References
Sharma, O. K., andBorek, E. 1973. Hormonal control of tRNA methyltransferases and of tRNA structure. Pages 85–102,in G. Weber (ed.) Advances in Enzyme Regulation, vol. 12, Pergamon Press, London.
Kerr, S. J., andHeady, J. E. 1973 Modulation of tRNA methyltransferase activity by competing enzyme systems. Pages 103–117, inG. Weber (ed.), Advances in Enzyme Regulation, vol. 12, Pergamon Press, London.
Sellinger, O. Z., Dainat, J., andSallas, C. E. 1978. The relationship of tRNA methylation to brain protein synthesis during cortical and cerebellar development. Pages 55–70,in S. Roberts, A. Lajtha andW. H. Gispen (eds.), Mechanism, Regulation and special functions of protein synthesis, Elsevier/North Holland Biomedical Press, Amsterdam.
Johnson, T. C., Mathews, R. A., andChou, L. 1974. tRNA methyltransferase activity in neonatal and mature mammalian neural tissue. J. Neurochem. 23:489–498.
Johnson, T. C., andChou, L. 1973. Level of amino acid acceptor activity of mouse brain tRNA during neural development. J. Neurochem. 20:405–414.
Harris, C. L., andMaas, J. W. 1974. Transfer RNA and the regulation of protein synthesis in rat cerebral cortex during neural development. J. Neurochem. 22:741–749.
Liu, D. S. H., andRichardson, A. 1977. Comparison of transfer ribonucleic acid aminoacylation by various regions of chicken brain. Experientia 33:601–602.
Trottier, B. 1974. La méthylation du tRNA: Etudein vitro sur le cerveau et le foie. Ph.D. Thesis, Laval University, Québec, Canada.
Elliott, K. A. C. 1969. The use of brain slices. Pages 103–114,in A. Lajtha (ed.), Handbook of Neurochemistry, vol. 2, Plenum Press, New York.
Tower, D. B. 1969. Inorganic constituents. Pages 1–24in A. Lajtha (ed.), Handbook of Neurochemistry, vol. 1, Plenum Press, New York.
Bharucha, A. D., Charbonneau, R., andMurthy, M. R. V. 1978. Aminoacylation of tRNAin vitro by homologous and heterologous systems derived from rat brain and liver. Can. Fed. Biol. Soc. 21:134.
Palmiter, R. D. 1973. Ovalabumin messenger ribonucleic acid translation. J. Biol. Chem. 248:2095–2106.
MacDonnell, P., Kenneth, H., Grouse, L., andGuroff, G. 1980. Brain nucleic acids. Pages 211–240,in S. Kumar (ed.), Biochemistry of Brain, Pergamon Press, New York.
Nau, F. 1976. The methylation of tRNA. Biochimie 58:629–645.
Nau, F. 1974. Differencesin vivo methylation patterns of Tyrosine and Histidine transfer ribonucleic acids from rat liver and novikoff hepatoma. Biochemistry 13:1105–1109.
Sheid, B., andPedrinan, L. 1977. Transfer RNA methylase activity in normal and dystrophic muscle. Biochem. Biophys. Res. Commun. 75:56–61.
Sheid, B., Wilson, S. M., andMorris, H. P. 1971. Transfer RNA methylase activity in normal rat liver and some Morris hepatomas. Cancer Res. 31:774–777.
Greengard, P. 1976. Possible role for cyclic nucleotides and phosphorylated membrane proteins in postsynaptic actions of neurotransmitters. Nature (London) 260:101–108.
Nathanson, J. A. 1977. Cyclic nucleotides and nervous system function. Physiol. Reviews 57:157–256.
Sharma, S. K., andTalwar, G. P. 1970. Action of cyclic adenosine 3′,5′-uptake and incorporation of uridine into ribonucleic acid in ovariectomized rat uterus. J. Biol. Chem. 245:1513–1519.
Juvenal, G. J., Pisarev, D. L. K., Crenovich, L., andPisarev, M. A. 1978. Role of neurotransmitters, prostaglandins and glucose on precursor incorporation into the RNA of thyroid slices. Acta Endocr. 87:776–785.
Bharucha, A. D., andElliott, K. A. C. 1974. Effects of electrically induced convulsions and transmitters substances on ribonucleic acid turnover in brain slices. Exp. Brain Res. 19:119–123.
Jost, J-P., andSahib, M. K. 1971. Role of cyclic adenosine 3′,5′-monosphosphate in the induction of hepatic enzymes. J. Biol. Chem. 246:1623–1629
Adiga, P. R., Murthy, P. N., andMcKenzie, J. M. 1971. Stimulation by thyrotropin, long-acting thyroid stimulator, and dibutyryl 3′,5′-adenosine monophosphate of protein and ribonucleic acid synthesis and ribonucleic acid polymerase activities in procine thyroid in vitro. Biochemistry, 10:702–710.
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Chaudhary, K.D., Carrier-Malhotra, L. & Murthy, M.R.V. Brain transfer RNA. Neurochem Res 7, 55–65 (1982). https://doi.org/10.1007/BF00965069
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DOI: https://doi.org/10.1007/BF00965069