Abstract
The distribution of UDP-galactose: ceramide galactosyltransferase (CGalT) was studied in subcellular fractions of rat forebrain during development using zonal centrifugation on linear gradients. Specialized subfractions: SN 1, a microsomal fraction, SN 4, a myelin-related fraction, and purified myelin were also used for this study. For comparison, two microsomal lipid synthesizing enzymes, a myelin-specific enzyme, 2′,3′-cyclic nucleotide 3′-phosphodiesterase and myelin proteins were measured in the same subfractions. UDP-glucose: ceramide glucosyltransferase and cerebroside sulfotransferase were confined to microsomes. CGalT was ferase and cerebroside sulfotransferase were confined to microsomes. CGalT was localized in microsomes, but also in myelin and myelin-related fractions. The developmental change in distribution of CGalT in adult animals toward myelin containing fractions could indicate that the replacement of galactosylceramide in compact myelin could be carried out in close proximity to compact myelin (mesaxon, paranodal loops) rather than in the distant oligodendrocyte perikaryon.
Similar content being viewed by others
References
Burkart, T., Siegrist, H. P., Herschkowitz, N. N., andWiesmann, U. N. 1977. 3′-Phosphoadenylsulfate: galactosylceramide 3′-sulfotransferase. An optimized assay in homogenates of developing brain. Biochim. Biophys. Acta 483:303–311.
Cestelli, A., White, F. V., andCostantino-Ceccarini, E. 1979. The use of liposomes as acceptors for the assay of lipid glycosyltransferases from rat brain. Biochim. Biophys. Acta 572:283–292.
Costantino-Ceccarini, E., andSuzuki, K. 1975. Evidence for the presence of UDP-galactose: ceramide galactosyltransferase in rat myelin. Brain Res. 93:358–362.
Costantino-Ceccarini, E., Cestelli, A., andDeVries, G. H. 1979. Characterization and developmental changes of UDP-galactose: ceramide galactosyltransferase in a rat CNS axolemma-enriched fraction. Differences and similarities of the enzyme associated with the microsomal and myelin fractions. J. Neurochem. 32:1175–1182.
Jungalwala, F. B. 1974. Synthesis and turnover of cerebroside sulfate of myelin in adult and developing rat brain. J. Lipid Res. 15:114–123.
Koul, O., Chou, K.-H., andJungalwala, F. B. 1980. UDP-galactose: ceramide galactosyltransferase in rat brain myelin subfractions during development. Biochem. J. 186:959–969.
Kurihara, T. andTsukada, Y. 1967. The regional and subcellular distribution of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in the central nervous system. J. Neurochem. 14:1167–1174.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Matthieu, J.-M., Honegger, P., Favrod, P., Gautier, E., andDolivo, M. 1979. Biochemical characterization of a myelin fraction isolated from rat brain aggregating cell cultures. J. Neurochem. 32:869–881.
Nescovic, N. M., Sarlieve, L. L., andMandel, P. 1973. Subcellular and submicrosomal distribution of glycolipidsynthesizing transferases in young rat brain. J. Neurochem. 20:1419–1430.
Norton, W. T. 1980. Myelin enzymes: indicators of non-insulating functions. Pages 64–75.in Boese, A. (ed.), Search for the cause of multiple sclerosis and other chronic diseases of the central nervous system, Verlag Chemie, Weinheim, FRG.
Sims, N. R., andCarnegie, P. R. 1978. 2′,3′-Cyclic nucleotide 3′-phosphodiesterase. Adv. Neurochem. 3:1–41.
Suzuki, K. 1980. Myelin-associated enzymes. Pages 333–347,in Baumann, N. (ed.), Elsevier/North-Holland Biomedical Press, Amsterdam, Neurological Mutations Affecting Myelination INSERM Symposium No. 14.
Waehneldt, T. V. 1975. Ontogenetic study of a myelin-derived fraction with 2′,3′-cyclic nucleotide 3′-phosphohydrolase activity higher than that of myelin. Biochem. J. 151:435–437.
Waehneldt, T. V. 1978. Density and protein profiles of myelin from two regions of young and adult rat CNS. Brain Res. Bull. 3:37–44.
Waehneldt, T. V., andLane, J. D. 1980. Dissociation of myelin from its “enzyme markers” during ontogeny. J. Neurochem. 35:566–573.
Waehneldt, T. V., Matthieu, J.-M., andNeuhoff, V. 1977. Characterization of a myelin-related fraction (SN 4) isolated from rat forebrain at two developmental stages. Brain Res. 138:29–43.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Costantino-Ceccarini, E., Waehneldt, T.V., Ginalski, H. et al. Distribution of lipid synthesizing enzymes, 2′,3′-cyclic nucleotide 3′-phosphodiesterase, and myelin proteins in rat forebrain subfractions during development. Neurochem Res 7, 1–12 (1982). https://doi.org/10.1007/BF00965064
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00965064