Abstract
After prolonged treatment of rats with lithium (pellets, 0.21% lithium carbonate, or 0.5 mg/ml lithium chloride in drinking water) for three months, the level of lithium in plasma was 0.87 meq/liter; in several brain regions, between 1.06–1.39 μeq/g wet weight. The content of sodium and potassium in the plasma was normal. The level of potassium in the brain regions tested increased by 13–30% and that of sodium by about 10%. Glycine levels increased significantly in all the regions (cerebral cortex, midbrain, cerebellum, and spinal cord). In the cerebellum GABA was also increased, while glutamine was decreased. In midbrain, apart from increases in glycine levels, alamine, valine, GABA and lysine were also increased. In the spinal cord, glutamic acid was also increased. Changes were largely in the putative neurotransmitters. Long-term treatment with lithium also influenced the high-affinity binding of [3H]spiperone in the cerebral cortex and corpus striatum. Two specific binding sites were found in both brain regions; the main change was the reduction in the lower affinity binding site (B max2).
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Fieve, R. R.. The clinical effects of lithium treatment. 1979. Trends in Neurochem. Sci. 2:5–8.
Wajda, I. J., Banay-Schwartz, M., Manigault, I., andLajtha, A. 1980. The effect of lithium and other ions on dopamine and opiate receptor binding in the striatum of rats. Trans. Am. Soc. Neurochem. 11:221.
Wajda, I. J., Banay-Schwartz, M., Manigault, I., andLajtha, A. 1981. Effect of lithium and sodium ions on opiate-and dopamine-receptor binding. Neurochem. Res. 6:321–331.
Wajda, I. J., Banay-Schwartz, M., Manigault, I., andLajtha, A. 1981. Chronic lithium treatment, the levels of ions and amino acids in brain, plasma and erythrocytes, and high-affinity (3H)naloxone binding. Trans. Am. Soc. Neurochem. 12:137.
Wajda, I. J., Banay-Schwartz, M., Manigault, I., andLajtha, A. 1981. Lithium: Ionic content of plasma and brain and high-affinity binding of (3H)naloxone in the brain of rats. In press.
Pert, A., Rosenblatt, J. E., Sivit, C. B., andBunney, W. E., Jr. 1978. Long term treatment with lithium prevents the development of dopamine receptor supersensitivity. Science. 201:171–172.
Rosenblatt, J. E., Pert, C. B., Tallman, J. F., Pert, A., andBunney, W. E., Jr. 1979. The effect of imipramine and lithium on alfa and beta-adrenergic receptor binding in rat brain. Brain Res. 160:186–191.
Rosenblatt, J. E., Pert, A., Layton, B., andBunney, W. E., Jr. 1980. Chronic lithium reduces (3H)spiroperidol binding in rat striatum. Eur. J. Pharmacol. 67:321–322.
Treiser, S., andKellar, K. J. 1979. Lithium effects on adrenergic receptor supersensitivity in rat brain. Eur. J. Pharmacol. 58:85–86.
Treiser, S., andKellar, K. J. 1980. Lithium: Effects on serotonin receptors in rat brain. Eur. J. Pharmacol. 64:183–185.
Maggi, A., andEnna, S. J. 1980. Regional alternatives in rat brain neurotransmitter system following lithium treatment. J. Neurochem. 34:888–892.
Seeman, P., Chan-Wong, M., Tedesco, J., andWong, K. 1975. Brain receptors for antipsychotic drugs and dopamine: Direct binding assays. Proc. Natl. Acad. Sci. USA 72:4376–4380.
Lowry, O. H., Rosebrough, N. J., Farr, L. A., andRandall, R. J. 1951. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193:265–275.
Banay-Schwartz, M., Zanchin, G., DeGuzman, T., andLajtha, A. 1979. The effect of corticosteroids on amino acid content of brain tissue preparations. Psychoneuroendocrinology 4:207–217.
Spring, G. K. 1979. Neurotoxicity with combined use of lithium and thioridazine. J. Clin. Psychiat. 40:135–138.
Ebadi, M. S., Simons, V. J., Hendrickson, M. J., andLacy, P. S. 1974. Pharmacokinetics of lithium and its regional distribution in rat brain. Eur. J. Pharmacol. 27:324–329.
Bradbury, M. W. B. 1971. Potassium homeostasis in cerebrospinal fluid. Pages 138–153,in Siesjo, B. K., andSorensen, S. C. (eds.), Ion homeostasis of the brain, Academic Press, New York.
Rosenblatt, S., Gaull, G. E., Chanley, J. D., Rosenthal, J. S., Smith, H., andSarcozi, L. 1979. Amino acid in bipolar affective disorders: Increased glycine levels in erythrocytes. Am. J. Psychiat. 136:672–674.
Deutcsh, S. I., Peselow, E. D., Banay-Schwartz, M., Gershon, S., Virgilio, J., Fieve, R. R., andRotrosen, J. 1981. Effect of lithium on glycine levels in patients with affective disorders. Am. J. Psychiat. 138:683–684.
Aprison, M. H., Davidoff, R. A., andWerman, A. 1970. Metabolic reactions in the nervous system. Pages 381–397,in Lajtha, A. (ed.), Handbook of Neurochemistry, Vol. 3., Plenum Press, New York.
Near, J. A., andMahler, H. R. 1981. Dopamine receptors in subcellular fractions from bovine caudate: Enrichment of [3H]spiperone binding in a postsynaptic membrane fraction. J. Neurochem. 36:1142–1151.
Friedman, E., andGershon, S., 1973. Effect of lithium on brain dopamine. Nature 243:520–521.
Corrodi, H., Fuxe, K., andShou, M. 1969. The effect of prolonged lithium adminstration on cerebral monoamine neurons in the rat. Life Sci. 8:643–651.
Ho, A. K. S., Loh, H. H., Craves, F., Hitzemann, R. J., andGershon, S. 1970. The effect of prolonged lithium treatment on the synthesis rate and turnover of monoamines in brain regions of rat. Europ. J. Pharmacol. 10:72–78.
Stein, D. N., Fieve, R. R., Neff, N. H., andCosta, E. 1969. The effect of lithium chloride administration on brain and heart norepinephrine turnover rates. Psychopharmacologia 14:315–320.
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Banay-Schwartz, M., Wajda, I.J., Manigault, I. et al. Lithium: Effect on [3H]spiperone binding, ionic content, and amino acid levels in the brain of rats. Neurochem Res 7, 179–189 (1982). https://doi.org/10.1007/BF00965056
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DOI: https://doi.org/10.1007/BF00965056