Neurochemical Research

, Volume 6, Issue 9, pp 1019–1033 | Cite as

High-affinity binding ofl-glutamate to chick retinal membranes

  • A. M. López-Colomé
Original Articles

Abstract

Binding ofl-[3H]glutamate to membranes from whole chick retina and from subcellular fractions enriched with photoreceptor terminals (P1), or terminals from the inner plexiform layer (P2) was studied. Na+-dependent and Na+-independent binding to these membranes was demonstrated. Na+-independent binding was stereospecific. Kinetic analysis of the binding process indicated a single high-affinity system (KB=0.55 μM) with a capacity of approximately 20 pmoles/mg protein in all the membrane fractions. [3H]Glutamate binding to P1 and P2 fractions was effectively displaced by several structural analogues of glutamate. Glutamate diethyl-ester appreciably displaced binding, whereas kainic acid did not displace bound glutamate. Data indicate the binding of [3H]glutamate to physiologically relevant receptors in the chick retina.

Keywords

Glutamate Retina Kinetic Analysis Membrane Fraction Structural Analogue 

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Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • A. M. López-Colomé
    • 1
  1. 1.Centro de Investigaciones en Fisiología CelularUniversidad Nacional Autónoma de MéxicoMéxico 20Mexico

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