Advertisement

Neurochemical Research

, Volume 5, Issue 5, pp 551–560 | Cite as

An improved apparatus for blood perfusion of the canine cerebral vasculature

  • Lester R. Drewes
Original Articles

Abstract

An improved perfusion apparatus is described which consists of a membrane oxygenator, roller pump, reservoir, heat exchanger, blood filter, and inert tubing. Heparinized blood may be used and is delivered at flow rates from 10 to 250 ml/min. Dogs are anesthetized with halothane and their cerebral arterial blood supply isolated by the method of Gilboe et al. (8). When the canine brain is perfused for 5 hr using the described apparatus, the rates of cerebral oxygen and glucose consumption are 5.19±0.12 ml/100 g/min and 39.9±6.5 μmol/100 g/min, respectively. Of the total glucose consumed by the brain, about 1/4 is contributed by the erythrocytes. An equivalent of about 9% of the consumed glucose is returned to the blood as lactate. Electron microscopic examination of cerebral cortex samples reveals no differences between 5-hr perfused brain and appropriate nonperfused controls. It is concluded that the apparatus is a useful system for organ perfusion and that the canine brain perfused by this method remains physiologically and metabolically active for at least 5 hr.

Keywords

Halothane Electron Microscopic Examination Perfuse Brain Cerebral Oxygen Organ Perfusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Apstein, C. S., Puchner, E., andBrachfeld, N. 1970. Improved automated lactate determination. Anal. Biochem. 38:20–34.PubMedGoogle Scholar
  2. 2.
    Drewes, L. R., Conway, W. P., andGilboe, D. D. 1970. Net amino acid transport between plasma and erythrocytes and perfused dog brain. Am. J. Physiol. 233:E320–E325.Google Scholar
  3. 3.
    Drewes, L. R., Frazin, L., andLevin, A. B. 1975. Blood flow in the isolated perfused dog brain: Normal oxygenation vs. anoxic anoxia. Physiologist 18:198.Google Scholar
  4. 4.
    Drewes, L. R., andGilboe, D. D. 1973. Glycolysis and the permeation of glucose and lactate in the isolated, perfused dog brain. J. Biol. Chem. 248:2489–2496.PubMedGoogle Scholar
  5. 5.
    Drewes, L. R., Gilboe, D. D., andBetz, A. L. 1973. Metabolic alterations in brain during anoxic-anoxia and subsequent recovery. Arch. Neurol. 29:385–390.PubMedGoogle Scholar
  6. 6.
    Elwyn, D. H., Launder, W. J., Parikh, H. C., andWise, E. M., Jr. 1972. Roles of plasma and erythrocytes in interorgan transport of amino acids in dogs. Am. J. Physiol. 222:1333–1342.PubMedGoogle Scholar
  7. 7.
    Fitzpatrick, J. H., Jr., Gilboe, D. D., Drewes, L. R., andBetz, A. L. 1976. Relationship of cerebral oxygen uptake to EEG frequency in isolated canine brain. Am. J. Physiol. 231:1840–1846.PubMedGoogle Scholar
  8. 8.
    Gilboe, D. D., Betz, A. L., andDrewes, L. R. 1975. Use of the isolated canine brain in studies of cerebral metabolism, metabolite transport, and cerebrovascular physiology, Pages 6–21,in Marks, N., andRodnight, R. (eds.), Research Methods in Neurochemistry, Vol. 3. Plenum Press, New York.Google Scholar
  9. 9.
    Laris, P. C. 1958. Permeability and utilization of glucose in mammalian erythrocytes. J. Cell. Comp. Physiol. 51:273–307.Google Scholar
  10. 10.
    Lee, P., Anvil, J., Grey, F. I., andSmith, M. 1976. 3-O-Methyl glucose transport in new-born and adult dog red cells. Fed. Proc. 35:780.Google Scholar
  11. 11.
    Michenfelder, J. D., andTheye, R. A. 1971. Effects of fentanyl, droperidol and innovar on canine cerebral metabolism and blood flow. Br. J. Anaesthesiol. 43:630–635.Google Scholar
  12. 12.
    Nemoto, E. M., Hoff, J. T., andSeveringhaus, J. W. 1974. Lactate uptake and metabolism by brain during hyperlactatemia and hypoglycemia. Stroke 5:48–53.PubMedGoogle Scholar
  13. 13.
    Reneman, R. S., Wellens, D., Jageneau, A., andStynen, L. 1974. Vertebral and carotid blood distribution in the brain of the dog and the cat. Cardiovasc. Res. 8:65–72.PubMedGoogle Scholar
  14. 14.
    Theye, R. A., andMichenfelder, J. D. 1968. The effects of nitrous oxide on canine cerebral metabolism. Anesthesiology 29:1119–1124.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • Lester R. Drewes
    • 1
  1. 1.Department of Biochemistry School of MedicineUniversity of Minnesota, DuluthDuluth

Personalised recommendations