Abstract
The present work describes the kinetics of enkephalin hydrolysis by plasma enzymes and the fragmentation pattern of both the parent peptide and of the first hydrolysis by-products. The degradation kinetics were followed by positive identification of the hydrolysis fragments by chromatographic methods, by amino acid analysis and by scintillation counting of tritium-labeled enkephalin. In addition, the results presented confirm the role of the low molecular weight plasma components in the control of the hydrolysis of the peripherally-released enkephalins.
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Roscetti, G., Possenti, R., Bassano, E. et al. Mechanisms of leu-enkephalin hydrolysis in human plasma. Neurochem Res 10, 1393–1404 (1985). https://doi.org/10.1007/BF00964980
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DOI: https://doi.org/10.1007/BF00964980