Neurochemical Research

, Volume 10, Issue 10, pp 1335–1341 | Cite as

Amino acid and monoamine transport in primary astroglial cultures from defined brain regions

  • Elisabeth Hansson
  • Peter Eriksson
  • Michael Nilsson
Original Articles


The uptake ofl-[3H]glutamate,l-[3H]aspartate, γ-[3H]aminobutric acid (GABA), [3H]dopamine,dl-[3H]norepinephrine and [3H]5-hydroxytryptamine (5-HT) was studied in astrocytes cultured from the cerebral cortex, striatum and brain stem of newborn rat and grown for 2 weeks in primary cultures. The astrocytes exhibited a high-affinityl-glutamate uptake withKm values ranging from 11 to 110 μM.Vmax values were 4.5 in cerebral cortex, 39.1 in striatum, and 0.4 in brain stem, nmol per mg cell protein per min. There was a less prominent high-affinity uptake ofl-aspartate withKm values from 88 to 187 μM.Vmax values were 7.4 in cerebral cortex, 37.1 in striatum, and 3.1 in brain stem, nmol per mg cell protein per min. The high-affinity GABA uptake exhibitedKm values ranging from 5 to 17 μM andVmax values were 0.01 for cerebral cortex, 0.04 for striatum, and 0.1 for brain stem, nmol per mg cell protein per min. No high-affinity, high-capacity uptake was found for the monoamines. The results demonstrate a heterogeneity among the astroglial cells cultivated from the different brain regions concerning the uptake capacity of amino acid neurotransmitters. Furthermore, amino acid transmitters and monoamines are taken up by the cells in different ways.


Dopamine Glutamate Cerebral Cortex Monoamine Brain Stem 
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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Elisabeth Hansson
    • 1
  • Peter Eriksson
    • 1
  • Michael Nilsson
    • 1
  1. 1.Institute of NeurobiologyUniversity of GöteborgGöteborgSweden

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