Advertisement

Neurochemical Research

, Volume 10, Issue 10, pp 1335–1341 | Cite as

Amino acid and monoamine transport in primary astroglial cultures from defined brain regions

  • Elisabeth Hansson
  • Peter Eriksson
  • Michael Nilsson
Original Articles

Abstract

The uptake ofl-[3H]glutamate,l-[3H]aspartate, γ-[3H]aminobutric acid (GABA), [3H]dopamine,dl-[3H]norepinephrine and [3H]5-hydroxytryptamine (5-HT) was studied in astrocytes cultured from the cerebral cortex, striatum and brain stem of newborn rat and grown for 2 weeks in primary cultures. The astrocytes exhibited a high-affinityl-glutamate uptake withKm values ranging from 11 to 110 μM.Vmax values were 4.5 in cerebral cortex, 39.1 in striatum, and 0.4 in brain stem, nmol per mg cell protein per min. There was a less prominent high-affinity uptake ofl-aspartate withKm values from 88 to 187 μM.Vmax values were 7.4 in cerebral cortex, 37.1 in striatum, and 3.1 in brain stem, nmol per mg cell protein per min. The high-affinity GABA uptake exhibitedKm values ranging from 5 to 17 μM andVmax values were 0.01 for cerebral cortex, 0.04 for striatum, and 0.1 for brain stem, nmol per mg cell protein per min. No high-affinity, high-capacity uptake was found for the monoamines. The results demonstrate a heterogeneity among the astroglial cells cultivated from the different brain regions concerning the uptake capacity of amino acid neurotransmitters. Furthermore, amino acid transmitters and monoamines are taken up by the cells in different ways.

Keywords

Dopamine Glutamate Cerebral Cortex Monoamine Brain Stem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Balcar, V. J., Mark, J., Borg, J., andMandel, P. 1979. High affinity uptake of γ-aminobutyric acid in cultured glial and neuronal cells. Neurochem. Res. 4:339–354.PubMedGoogle Scholar
  2. 2.
    Borg, J., Ramaharobandro, N., Mark, J., andMandel, P. 1980. Changes in the uptake of GABA and taurine during neuronal and glial maturation. J. Neurochem. 34:1113–1122.PubMedGoogle Scholar
  3. 3.
    De Feudis, F. V. 1975. Amino acids as central neurotransmitters. Ann. Rev. Pharmacol. 15:105–130.PubMedGoogle Scholar
  4. 4.
    Drejer, J., Larsson, O. M., andSchousboe, A. 1982. Characterization of L-glutamate uptake into and release from astrocytes and neurons cultured from different brain regions. Exp. Brain Res. 47:259–269.PubMedGoogle Scholar
  5. 5.
    Drejer, J., Larsson, O. M., andSchousboe, A. 1983. Characterization of uptake and release processes ford- andl-aspartate in primary cultures of astrocytes and cerebellar granule cells. Neurochem. Res. 8:231–243.PubMedGoogle Scholar
  6. 6.
    Hallermayer, K., andHamprecht, B. 1984. Cellular heterogeneity in primary cultures of brain cells revealed by immunocytochemical localization of glutamine synthetase. Brain Research 295:1–11.PubMedGoogle Scholar
  7. 7.
    Hansson, E. 1983. Accumulation of putative amino acid neurotransmitters, monoamines and D-ala2met-enkephalinamide in primary astroglial cultures from various brain areas, visualized by autoradiography. Brain Research 289:189–196.PubMedGoogle Scholar
  8. 8.
    Hansson, E. 1984. Enzyme activities of monoamine oxidase, cathechol-O-methyltransferase and γ-aminobutyric acid transaminase in primary astroglial cultures and adult rat brain from different brain regions. Neurochem. Res. 9:45–57.PubMedGoogle Scholar
  9. 9.
    Hansson, E. 1985. Transport of monoamine and amino acid neurotransmitters by primary astroglial cultures. Neurochem. Res. 10:591–599.PubMedGoogle Scholar
  10. 10.
    Hansson, E. 1985. Primary cultures from defined brain areas. Part 2. Effects of seeding time on the development of β-adrenergic and dopamine stimulated cAMP-activity during cultivation. Brain Research (in press)Google Scholar
  11. 11.
    Hansson, E. 1985. Primary cultures from defined brain areas. Part 3. Effects of seeding time onl-[3H]glutamate transport and glutamine synthetase activity. Submitted.Google Scholar
  12. 12.
    Hansson, E., Isacsson, H., andSellström, Å. 1984. Characteristics of dopamine and GABA transport in primary cultures of astroglial cells. Acta Physiol. Scand. 121:333–341.PubMedGoogle Scholar
  13. 13.
    Hansson, E., Rönnbäck, L., andSellström, Å. 1984. Is there a “dopaminergic glial cell”? Neurochem. Res. 9:679–689.PubMedGoogle Scholar
  14. 14.
    Hansson, E., Rönnbäck, L., Persson, L. I., Lowenthal, A., Noppe, M., Alling, C., andKarlsson, B. 1984. Cellular composition of primary cultures from cerebral cortex, striatum, hippocampus, brain stem and cerebellum. Brain Research 300:9–18.PubMedGoogle Scholar
  15. 15.
    Hansson, E. Rönnbäck, L., Lowenthal, A., andNoppe, M. 1985. Primary cultures from defined brain areas. Effects of seeding time on cell growth, astroglial content and protein synthesis. Brain Research (in press)Google Scholar
  16. 16.
    Henn, F. A., Goldstein, M. N., andHamberger, A. 1974. Uptake of the neurotransmitter candidate glutamate by glia. Nature (Lond.) 249:663–664.Google Scholar
  17. 17.
    Hertz, L., Bock, E., andSchousboe, A. 1978. GFA content, glutamate uptake and activity of glutamate metabolizing enzymes in differentiating mouse astrocytes in primary cultures. Dev. Neurosci. 1:226–238.Google Scholar
  18. 18.
    Hösli, E., Bucher, U. M., andHösli, L. 1975. Uptake of3H-noradrenaline and3H-5-hydroxytryptamine in cultured rat brain stem. Experientia 31:354–356.PubMedGoogle Scholar
  19. 19.
    Hösli, E., andHösli, L. 1976. Autoradiographic studies on the uptake of3H-noradrenaline and3H-GABA in cultured rat cerebellum. Exp. Brain Res. 36:319–324.Google Scholar
  20. 20.
    Hösli, E., andHösli, L. 1978. Action and uptake of neurotransmitters in CNS tissue culture. Rev. Physiol. Biochem. Pharmacol. 81:135–188.PubMedGoogle Scholar
  21. 21.
    Iversen, L. L., andNeal, M. J. 1968. The uptake of (3H)-GABA by slices of rat cerebral cortex. J. Neurochem. 15:1141–1149.PubMedGoogle Scholar
  22. 22.
    Lowry, O. H., Roserbrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.PubMedGoogle Scholar
  23. 23.
    Magistretti, P. J., Manthorpe, M., Bloom, F. E., andVaron, S. 1983. Functional receptors for vasoactive intestinal polypeptide in cultured astroglia from neonatal rat brain. Regulatory Peptides 6:71–80.PubMedGoogle Scholar
  24. 24.
    Narumi, S., Kimelberg, H. K., andBourke, R. S. 1978. Effects of norepinephrine on the morphology and some enzyme activities of primary monolayer cultures from rat brain. J. Neurochem. 31:1479–1490.PubMedGoogle Scholar
  25. 25.
    Pelton, E. W. II, Kimelberg, H. K., Shipherd, S. V., andBourke, R. S. 1981. Dopamine and norepinephrine uptake and metabolism by astroglial cells in culture. Life Sci. 28:1655–1663.PubMedGoogle Scholar
  26. 26.
    Rougon, G., Noble, M., andMudge, A. W. 1983. Neuropeptides modulate the β-adrenergic response of purified astrocytes in vitro. Nature 305:715–717.PubMedGoogle Scholar
  27. 27.
    Schousboe, A., Hertz, L., andSvenneby, G. 1977. Uptake and metabolism of GABA in astrocytes cultured from dissociated mouse brain hemispheres. Neurochem. Res. 2:217–229.Google Scholar
  28. 28.
    Schousboe, A., Svenneby, G., andHertz, L. 1977. Uptake and metabolism of glutamate in astrocytes cultured from dissociated mouse brain hemispheres, J. Neurochem. 29:999–1005.PubMedGoogle Scholar
  29. 29.
    Sensenbrenner, M., Devilliers, G., Bock, E., andPorte, A. 1980. Biochemical and ultrastructural studies of cultured rat astroglial cells. Differentiation 17:51–61.PubMedGoogle Scholar
  30. 30.
    Snyder, S. H., Kuhar, M. J., Green, A. I., Coyle, J. T., andShaskan, E. G. 1970. Uptake and subcellular localization of neurotransmitters in the brain. Int. Rev. Neurobiol. 13:127–158.Google Scholar
  31. 31.
    Van Calker, D., Müller, M., andHamprecht, B. 1980. Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells. Proc. Nat. Acad. Sci. (U.S.A.) 77:6907–6911.Google Scholar
  32. 32.
    Weiler, C. T., Nyström, B., andHamberger, A. 1979. Characteristics of glutamine vs. glutamate transport in isolated glia and synaptosomes. J. Neurochem. 32:559–565.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Elisabeth Hansson
    • 1
  • Peter Eriksson
    • 1
  • Michael Nilsson
    • 1
  1. 1.Institute of NeurobiologyUniversity of GöteborgGöteborgSweden

Personalised recommendations