Abstract
The supernatant fraction of centrifuged homogenate of brain tissue contains material that inhibits the saturable binding of [3H]cocaine to crude mouse brain membranes. This material was subjected to heat treatment to remove protein; further purification was achieved by filtering through an Amicon UM-10 membrane ultrafilter and gel filtration of the ultrafiltrate on Sephadex G-25. Sensitivity to acid hydrolysis and peptidase action indicates that the inhibitory activity resides in peptide material with a low molecular weight. The partially purified inhibitor has similar effects to that of cocaine on the specific binding of various ligands to opiate and nonopiate receptors in mouse brain membranes.
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Reith, M.E.A., Sershen, H. & Lajtha, A. Endogenous peptide(s) inhibiting [3H]cocaine binding in mouse brain. Neurochem Res 5, 1291–1299 (1980). https://doi.org/10.1007/BF00964964
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DOI: https://doi.org/10.1007/BF00964964