Abstract
Stereospecific [3H]etorphine binding sites are present in neuronal-enriched cell cultures dissociated from 7-day-old chick embryonic brain. Moreover, binding was regulated by both ions and GTP in a manner similar to that of in vivo brain tissue. When cultures were exposed to N-LAAM (10−6M) from day 6 to day 7 or 8 and assayed for binding at day 8,B max was decreased andK D was increased. These findings support our view that primary neuronal cultures are a suitable model with which to study interactions of drugs with opiate receptors.
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References
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Gibson, D.A., Vernadakis, A. Effects of N-LAAM on [3H]etorphine binding in neuronal-enriched cell cultures. Neurochem Res 8, 1197–1202 (1983). https://doi.org/10.1007/BF00964933
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DOI: https://doi.org/10.1007/BF00964933