Abstract
The high affinity uptake of GABA and glycine by synaptosomal preparations of rabbit hippocampus and spinal cord has been characterized. The high affinity uptake by such preparations from rabbits in which chronic neurofibrillary changes had been induced with aluminum chloride was measured and found to be reduced. The reduction was the greatest when the number of neurons affected was the greatest. These results suggest that normal neurotransmitter function is considerably disturbed in neurons affected with neurofibrillary changes.
Similar content being viewed by others
References
Iqbal, K., Wisniewski, H. M., Grundke-Iqbal, I., andTerry, R. D. 1977. Neurofibrillary pathology: An update. Pages 209–227,in Nandy, K., andSherwin, I. (eds.), The Aging Brain and Senile Dementia, Plenum Press, New York.
Wisniewski, H. M., andSoifer, D. 1979. Neurofibrillary pathology: Current status and research prospectives. Mech. Aging & Development 9:119–142.
Pope, A., Hess, H. H., andLewin, E. 1965. Microchemical pathology of the cerebral cortex in pre-senile dementias. Trans. Amer. Neurol. Assoc. 89:15–16.
Davies, P., andMaloney, A. J. F. 1976. Selective loss of central cholinergic neurons in Alzheimer's disease. Lancet ii:1403.
Perry, E. K., Perry, R. H., Blessed, G., andTomlinson, B. E. 1977. Necropsy evidence of central cholinergic deficits in senile dementia. Lancet i:189.
Perry, E. K., Tomlinson, B. E., Blessed, G., Bergmann, K., Gibson, P. H., andPerry, R. H. 1978. Correlation of cholinergic abnormalities with senile placques and mental test scores in senile dementia. Brit. Med. J. 2:1457–1459.
White, P., Goodhardt, M. J., Keet, J. P., Hiley, C. R., Carrasco, L. H., Williams, J. E., andBowen, D. M. 1977. Neocortical cholinergic neurons in elderly people. Lancet i:668–671.
Davies, P. 1978. Biochemical changes in alzheimer's disease-senile dementia: neurotransmitters in senile dementia of the Alzheimer's type. Pages 154–160,in Katzman, R. (ed.), Congenital and Acquired Cognitive Disorders, Raven Press, New York.
Davies, P. 1979. Neurotransmitter-related enzymes in senile dementia of the Alzheimer type. Brain Res. 171:319–327.
Rossor, M. N., Garrett, N. J., Johnson, A. L., Mountjoy, C. Q., Roth, M., andIversen, L. L. 1982. A post-mortem study of the cholinergic and GABA systems in senile dementia. Brain 105:313–330.
Whitehouse, P. J., Price, D. L., Struble, R. G., Clark, A. W., Coyle, J. T., andDelong, M. R. 1982. Alzheimer's disease and senile dementia: Loss of neurons in the basal forebrain. Science 215:1237–1239.
Bowen, D. M., Smith, C. B., White, P., andDavison, A. N. 1976. Neurotransmitter-related enzymes and indices of hypoxia in senile dementia and other abiotrophies. Brain 99:459–496.
Reisine, T. D., Yamamura, H. J., Bird, E. D., Spokes, E., andEnna, S. J. 1978. Pre-and postsynaptic neurochemical alterations in Alzheimer's disese. Brain 159:477–481.
Benton, J. S., Bowen, D. M., Allen, S. J., Haan, E. A., Davison, A. N., Neary, D., Murphy, R. P., andSnowden, J. S. 1982. Alzheimer's disease as a disorder of isodendritic core. Lancet i:456.
Wisniewski, H. M., Sturman, J. A., andShek, J. W. 1980. Aluminum chloride induced neurofibrillary changes in the developing rabbit: A chronic animal model. Ann. Neurol. 8:479–490.
Rabe, A., Lee, M. H., Shek, J., andWisniewski, H. M. 1982. Learning deficit in immature rabbits with aluminum induced neurofibrillary changes. Exp. Neurol. 76:441–446.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Quinn, M. R., andCagan, R. H. 1982. High specific binding of [3H]GABA and [3H]muscimol to membranes from dendrodendritic synaptosomes of the rat olfactory bulb. J. Neurochem. 39:1381–1386.
Schon, F., andKelly, J. S. 1975. Selective uptake of [3H]β-alanine by glia: associated with the glial uptake system for GABA. Brain Res. 86:243–257.
Simon, J. R., andMartin, D. L. 1973. The effects ofL-2, 4-diaminobutyric acid on the uptake of gamma-aminobutyric acid by a synaptosomal fraction from rat brain. Arch. Biochem. Biophys. 157:348–355.
Johnston, G. A. R., Stephanson, A. L., andTwitchin, B. 1976. Uptake and release of nipecotic acid by rat brain slices. J. Neurochem. 26:83–87.
Johnston, G. A. R., Krogsgaard-Larsen, P., Stephanson, A. L., andTwitchin, B. 1976. Inhibition of the uptake of GABA and related amino acids in rat brain slices by the optical isomers of nipecotic acid. J. Neurochem. 26:1029–1032.
Takagaki, G. 1976. Characteristics of the uptake and release of glutamic acid in synaptosomes from rat cerebral cortex. Adv. Exptl. Biol. Med. 69:307–317.
Hetnarski, B., Wisniewski, H. M., Iqbal, K., Dziedzic, J. D., andLajtha, A. 1980. Central cholinergic activity in aluminum-induced neurofibrillary degeneration. Ann. Neurol. 7:489–490.
Wisniewski, H. M., Shek, J. W., andSturman, J. A. 1982. Aluminum-induced neurofibrillary changes in axons and dendrites. Trans. Amer. Soc. Neurochem. 13:206.
Troncoso, J. C., Price, D. L., Griffin, J. W., andParhad, I. M. 1982. Neurofibrillary axonal pathology in aluminum intoxication. Ann. Neurol. 12:278–283.
Wisniewski, H. M., Sturman, J. A., andShek, J. W. 1982. Chronic model of neurofibrillary changes induced in mature rabbits by metallic aluminum. Neurobiol. Aging 3:11–22.
Pradhan, S. N. 1980. Central neurotransmitters and aging. Life Sci. 26:1643–1656.
Marchbanks, R. M. 1982. Biochemistry of Alzheimer's Dementia. J. Neurochem. 39:9–15.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sturman, J.A., Wisniewski, H.M. & Shek, J.W. High affinity uptake of GABA and glycine by rabbits with aluminum-induced neurofibrillary changes. Neurochem Res 8, 1097–1109 (1983). https://doi.org/10.1007/BF00964925
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00964925