Skip to main content
SpringerLink
Log in

Development of muscarinic cholinergic receptor binding in the visual system of monocularly deprived and dark reared rats

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

In 25 day old rats monocularly deprived by unilateral eyelid suture on postnatal day 10 (MD), [3H]quinuclidinyl benzylate (3H-QNB) binding was significantly reduced in the visual cortex (VC) of both sides, but elevated in both superior colliculi (SC). Muscarinic receptor binding in the frontal cortex (FC), a non-visual brain area, in the lateral geniculate nucleus (LGN), and in the retina was not affected. In 25 day old rats raised in complete darkness from birth (DR) similar changes in3H-QNB binding were found in VC and SC. However, binding levels were also decreased in the FC and significantly increased in the retina. In adult (6 month old) MD and DR rats the differences in3H-QNB binding as compared to age-matched controls had disappeared completely in all visual brain areas studied. Detailed Scatchard analyses indicate that the alterations in the3H-QNB binding were due to changes in receptor number only.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gudden, B. von 1870. Experimentaluntersuchungen über das peripherische und centrale Nervensystem. Arch. Psych. Nervenkrh. 2:693–723.

    Google Scholar 

  2. Pettigrew, J. D. 1978. The paradox of the critical period for striate cortex. Pages 311–330,in Cotman, C. W. (ed.), Neuronal plasticity, Raven Press, New York.

    Google Scholar 

  3. Bigl, V., andBiesold, D. 1978. Sensory deprivation and brain development. Pages 199–215,in Neuhoff, V. (ed.), Proceedings of the European Society for Neurochemistry, Verlag Chemie, Weinheim.

    Google Scholar 

  4. Globus, A. 1975. Brain morphology as a function of presynaptic morphology and activity. Pages 9–91,in Riesen, A. H. (ed.), The developmental neuropsychology of sensory deprivation, Academic Press, New York.

    Google Scholar 

  5. Chow, K. L. 1973. Neuronal changes in the visual system following visual deprivation. Pages 599–627,in Jung, R. (ed.), Handbook of sensory physiology, vol. VII/3. Central processing of visual information, A: Integrative function and comparative data, Springer Verlag, Berlin.

    Google Scholar 

  6. Barlowe, H. B. 1975. Visual experience and cortical development. Nature 258:199–204.

    Google Scholar 

  7. Lund, J. S., andLund, R. D. 1972. The effects of varying periods of visual deprivation on synaptogenesis in the superior colliculus of the rat. Brain Res. 42:21–32.

    Google Scholar 

  8. Winfield, D. A., Headon, M. D., andPowell, T. P. S. 1976. Postnatal development of the synaptic organisation of the lateral geniculate nucleus in the kitten with unilateral eyelid closure. Nature 263:591–594.

    Google Scholar 

  9. Vrensen, G. 1978. Ontogenesis of the visual cortex of rabbit and the effect of visual deprivation. Progr. Brain Res. 48:231–244.

    Google Scholar 

  10. Blakemore, C., andHillman, P. 1977. An attempt to assess the effects of monocular deprivation and strabismus on synaptic efficiency in the kitten's visual cortex. Exp. Brain Res. 30:187–202.

    Google Scholar 

  11. Guillery, R. W. 1974. On structural changes that can be produced experimentally in the mammalian visual pathways. Pages 299–326,in Bellairs, R., andGray, E. G. (eds.), Assays on the nervous system, Clarendon Press, Oxford.

    Google Scholar 

  12. Kasamatsu, T., andPettigrew, J. D. 1976. Depletion of brain catecholamines: failure of ocular dominance shift after monocular occlusion in kittens. Science 194:206–209.

    Google Scholar 

  13. Pettigrew, J. D., andKasamatsu, T. 1978. Local perfusion of noradrenaline maintains visual cortical plasticity. Nature 271:761–763.

    Google Scholar 

  14. Burgoyne, R. D., Schliebs, R., andBigl, V. 1980. The effect of visual deprivation on β-adrenergic receptors in the visual centres of the rat brain. Biochem. Soc. Trans. 8:623–624.

    Google Scholar 

  15. Schliebs, R., Burgoyne, R. D., andBigl, V. 1982. The effect of visual deprivation on β-adrenergic receptors in the visual centres of the rat brain. J. Neurochem. 38:1038–1043.

    Google Scholar 

  16. Singer, W. 1977. Control of thalamic transmission by corticofugal and ascending reticular pathways in the visual system. Physiol. Rev. 57:386–420.

    Google Scholar 

  17. Wenk, H., Bigl, V., andMeyer, U. 1980. Cholinergic projections from magnocellular nuclei of the basal forebrain to cortical areas in rats. Brain Res. Rev. 2:295–316.

    Google Scholar 

  18. Gorry, J. D. 1963. Studies on the comparative anatomy of the ganglion basale of Meynert. Acta. Anat. (Basel) 55:51–101.

    Google Scholar 

  19. Le Conte, G., Casamenti, F., Bigl, V., Milaneschi, E., andPepeu, G. 1981. Effect of magnocellular forebrain nuclei lesions on acetylcholine output from the cerebral cortex, electrocorticogram and behaviour. Arch. Ital. Biol., in press.

  20. Bigl, V., Biesold, D., andWeisz, K. 1974. The influence of functional alteration on monoamine oxidase and catechol-O-methyl-transferase in the visual pathway of rats. J. Neurochem. 22:505–509.

    Google Scholar 

  21. Yamamura, H. I., andSnyder, S. H. 1974. Muscarinic cholinergic binding in rat brain. Proc. Natl. Acad. Sci. USA 71:1725–1729.

    Google Scholar 

  22. Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  23. Scatchard, G. 1949. The attractions of proteins for small molecules and ions. Ann. N.Y. Acad. Sci. 51:660–672.

    Google Scholar 

  24. Rosenthal, H. E. 1967. Graphic method for the determination and presentation of binding parameters in a complex system. Anal. Biochem. 20:525–532.

    Google Scholar 

  25. Boeynaems, J. M., andDumont, J. E. 1980. Outlines of receptor theory. Elsevier/North-Holland Biomedical Press, Amsterdam.

    Google Scholar 

  26. Bennett, J. P. 1978. Pages 57–90,in Yamamura, H. I., Enna, S. J., andKuhar, M. J. (eds.), Neurotransmitter receptor binding, Raven Press, New York.

    Google Scholar 

  27. Maelicke, A., Fulpius, R. W., Klett, R. P., andReich, E. 1977. Acetylcholine receptor. Responses to drug binding. J. Biol. Chem. 252:4811–4830.

    Google Scholar 

  28. Schliebs, R., andBigl, V. 1982. Derivation of all Scatchard plot parameters in radioligand binding studies by assessment of total binding alone. J. Neurosci. Methods, submitted.

  29. Hulme, E. C., Birdsall, N. J. M., Burgen, A. S. V., andMehta, P. 1978. The binding of antagonists to brain muscarinic receptors. Mol. Pharmacol. 14:737–750.

    Google Scholar 

  30. Skangiel-Kramska, J. 1980. Biochemical approach to the studies on plasticity of cerebral cortex of visually deprived cats. Pages 353–378,in Brzin, M., Sket, D., andBachelard, H. (eds.), Synaptic constituents in healt and disease. Mladinska knjiga —Pergamon Press, Ljubljana-Oxford.

    Google Scholar 

  31. Bigl, V., andSchober, W. 1977. Cholinergic transmission in subcortical and cortical visual centres of rats: no evidence for the involvement of primary optic system. Exp. Brain Res. 27:211–219.

    Google Scholar 

  32. Potempska, A., Skangiel-Kramska, J., andKossut, M. 1979. Development of cholinergic enzymes and adenosinetriphosphatase activity of optic system of cats in normal and restricted visual input conditions. Dev. Neurosci. 2:38–45.

    Google Scholar 

  33. Domino, E. F., Krause, R. R., andBowers, J. 1973. Regional distribution of some enzymes involved with putative neurotransmitters in the human visual system. Brain Res. 58:179–189.

    Google Scholar 

  34. Maletta, G. J., andTimiras, P. S. 1968. Choline acetyltransferase activity and total protein content in selected optic areas of the rat after complete light-deprivation during CNS-development. J. Neurochem. 15:787–793.

    Google Scholar 

  35. Maletta, G. J., andTimiras, P. S. 1967. Acetylcholinesterase activity in optic structures after complete light deprivation from birth. Exp. Neurol. 19:513–518.

    Google Scholar 

  36. Reisine, T. D., Nagy, J. I., Beaumont, K., Fibiger, H. C., andYamamura, H. I. 1979. The localization of receptor binding sites in the substantia nigra and striatum of the rat. Brain Res. 177:241–252.

    Google Scholar 

  37. Kobayashi, R. M., Palkovits, M., Hruska, R., Rothschild, R., andYamamura, H. I. 1978. Regional distribution of muscarinic cholinergic receptors in rat brain. Brain Res. 154:13–23.

    Google Scholar 

  38. Sugiyama, H., Daniels, M. P., andNirenberg, M. 1977. Muscarinic acetylcholine receptors of the developing retina. Proc. Natl. Acad. Sci. USA 74:5524–5528.

    Google Scholar 

  39. Hruska, R. E., White, R., Azari, J., andYamamura, H. I. 1978. Muscarinic cholinergic receptors in mammalian retina. Brain Res. 148:493–498.

    Google Scholar 

  40. Aronstam, R. S., Kellogg, C., andAbood, L. G. 1979. Development of muscarinic cholinergic receptors in inbred strains of mice. Brain Res. 162:231–241.

    Google Scholar 

  41. Kuhar, M. J., Birdsall, N. J. M., Burgen, A. S. V., andHulme, E. C. 1980. Ontogeny of muscarinic receptors in rat brain. Brain Res. 184:375–383.

    Google Scholar 

  42. Brooksbank, B. W. L., Martinez, M. M., Atkinson, D. J., andBalasz, R. 1978. Biochemical development of the human brain. Dev. Neurosci. 1:267–284.

    Google Scholar 

  43. Coyle, J. T., andYamamura, H. I. 1976. Neurochemical aspects of ontogenesis of cholinergic neurons in the rat brain. Brain Res. 118:429–440.

    Google Scholar 

  44. Enna, S. J., Yamamura, H. I., andSnyder, S. H. 1976. Development of muscarinic cholinergic and GABA receptor binding in chick embryo brain. Brain Res. 101:177–183.

    Google Scholar 

  45. Yavin, E., andHarel, S. 1979. Muscarinic binding sites in the developing rabbit brain. FEBS Lett. 97:151–154.

    Google Scholar 

  46. Morin, A. M., andWasterlain, C. G. 1980. Aging and rat brain muscarinic receptors as measured by quinucleidinyl benzylate binding. Neurochem. Res. 5:301–308.

    Google Scholar 

  47. Dudai, Y., andYavin, E. 1978. Ontogenesis of muscarinic receptors and acetylcholinesterase in differentiating rat cerebral cells in culture. Brain Res. 155:368–373.

    Google Scholar 

  48. Bigl, V., Schliebs, R., Burgoyne, R. D., Kunert, E., Deutschmann, B., andBiesold, D. 1982. Biochemical studies in monocularly deprived rats. Pages 427–435in Marsan, C. A., andMatthies, H. (eds.), Neuronal plasticity and memory formation, Raven Press, New York.

    Google Scholar 

  49. Schober, W. 1975. Die primären optischen Projektionen bei Albinoratten und pigmentierten Ratten. Anat. Anz. 137:257–286.

    Google Scholar 

  50. Rothblat, L. A., Schwartz, M. L., andKasdan, P. M. 1978. Monocular deprivation in the rat: evidence for an age-related defect in visual behaviour. Brain Res. 158:456–460.

    Google Scholar 

  51. Kunert, E., andBigl, V. 1980. Postnatal development of parameters of GABA metabolism in the visual system of normal and visually deprived rats. Pages 375–376,in Di Benedetta, C., Balasz, R., Gombos, G., andPorcelatti, G. (eds.), Multidisciplinary approach to brain development. Elsevier/North-Holland Biomedical Press, Amsterdam.

    Google Scholar 

  52. Bigl, V., andButcher, L. L. 1981. Topography of the cholinergic innervation of the cerebral cortex in rat. Brain Res. Bull. submitted.

  53. Henderson, Z. 1981. A projection from acetylcholinesterase-containing neurones in the diagonal band to the occipital cortex of the rat. Neuroscience 6:1081–1088.

    Google Scholar 

  54. Shute, C. C. D., andLewis, P. R. 1967. The ascending cholinergic reticular system: neocortical, olfactory and subcortical projections. Brain Res. 90:497–520.

    Google Scholar 

  55. Bigl, V., Wenk, H., Meyer, U., andLüth, H.-J. 1979. Cholinergic mechanisms in the visual system of rat. Progr. Brain Res. 49:472.

    Google Scholar 

  56. Yamamura, H. I., andSnyder, S. H. 1974. Postsynaptic localization of muscarinic cholinergic receptor binding in rat hippocampus. Brain Res. 78:320–326.

    Google Scholar 

  57. Gurwitz, D., Kloog, Y., Egozi, Y., andSokolovsky, M. 1980. Central muscarinic receptor degeneration following 6-hydroxydopamine lesion in mice. Life Sci. 26:79–84.

    Google Scholar 

  58. Nordström, Ö, andBartfai, T. 1980. Muscarinic autoreceptor regulates acetylcholine release in rat hippocampus: in vitro evidence. Acta Physiol. 108:347–353.

    Google Scholar 

  59. Rose, S. P. R., andStewart, M. 1978. Transient increase in muscarinic acetylcholine receptor and acetylcholinesterase in visual cortex on first exposure of dark-reared rats to light. Nature 271:169–170.

    Google Scholar 

  60. Fleming, W. W., McPhillips, J. J., andWestfall, D. P. 1973. Postjunctional supersensitivity and subsensitivity of excitable tissues to drugs. Ergebn. Physiol. 68:55–119.

    Google Scholar 

  61. Schiller, G. D. 1979. Reduced binding of3H-quinuclidinyl benzylate associated with chronically low acetylcholinesterase activity. Life Sci. 24:1159–1164.

    Google Scholar 

  62. Gazit, H., Silman, I., andDudai, Y. 1979. Administration of an organophosphate causes a decrease in muscarinic receptor levels in rat brain. Brain Res. 174:351–356.

    Google Scholar 

  63. Ben-Barak, J., andDudai, Y. 1980. Scopolamine induces an increase in muscarinic receptor level in rat hippocampus. Brain Res. 193:309–313.

    Google Scholar 

  64. Siman, R. G., andKlein, W. L. 1979. Cholinergic activity regulates muscarinic receptors in central nervous system cultures. Proc. Natl. Acad. Sci. USA 76:4141–4145.

    Google Scholar 

  65. Gilbert, R. F. T., Hanley, M. R., andIversen, L. L. 1979.3H-Quinuclidinyl benzylate binding to muscarinic receptors in rat brain: comparison of results from intact brain slices and homogenates. Br. J. Pharmacol. 65:451–456.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Paul Flechsig Institute for Brain Research Dept. of Neurochemistry, Karl Marx University, Leipzig, G.D.R.

    Reinhard Schliebs, Volker Bigl & Dietmar Biesold

Authors
  1. Reinhard Schliebs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Volker Bigl
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dietmar Biesold
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This paper is dedicated to Dr. Derek Richter on his seventy-fifth birthday.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schliebs, R., Bigl, V. & Biesold, D. Development of muscarinic cholinergic receptor binding in the visual system of monocularly deprived and dark reared rats. Neurochem Res 7, 1181–1198 (1982). https://doi.org/10.1007/BF00964894

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00964894

Keywords

Search

Navigation