Neurochemical Research

, Volume 10, Issue 9, pp 1299–1310 | Cite as

Effects of glycoprotein synthesis inhibitor on myelination in rat cerebellum

  • Shinichi Kohsaka
  • Koichi Mita
  • Haruhiko Suda
  • Masayuki Matsuyama
  • Yasuzo Tsukada
Original Articles
  • 24 Downloads

Abstract

Effects of a glycoprotein synthesis inhibitor on myelination were investigated in rat cerebellum. The glycoprotein synthesis inhibitor, tunicamycin (TM), was injected intracranially into newborn rats. The activity of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) in the cerebellum was significantly reduced in 2-week-old animals and was restored to the normal level by age 3 weeks. When TM was injected into newborn rats every 3–4 days for a total of 6 times, CNPase activity was still low at 3 and 4 weeks. Immunohistochemical stainings for CNPase and myelin-associated glycoprotein (MAG) were performed on paraffin sections of multiple-TM-injected cerebellum at 3 weeks. The intensity of the staining with MAG antiserum in the white matter was clearly decreased in TM-treated cerebellum compared with the control. The myelin in the granule cell layer was poorly stained with CNPase antiserum in TM-treated cerebellum. Subcellular fractionation was carried out and the CNPase activity in each fraction was measured. The CNPase activity in the myelin fraction (P2A) from the TM-treated cerebellum was significantly lower than that in the control. In contrast, the activity in the synaptosomal (P2B) and microsomal (P3) fractions from the multiple-TM-injected cerebellum was higher than in those from the controls. Polyacrylamide gel electrophoretic patterns of the P2A fractions were analyzed. The P2A fraction from TM-treated cerebellum contained less Wolfgram protein than the control. These results suggest that glycoprotein synthesis plays certain roles in myelination in the central nervous system.

Keywords

White Matter Paraffin Immunohistochemical Staining Granule Cell Phosphodiesterase 

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Shinichi Kohsaka
    • 1
  • Koichi Mita
    • 1
  • Haruhiko Suda
    • 1
  • Masayuki Matsuyama
    • 1
  • Yasuzo Tsukada
    • 1
  1. 1.Department of Physiology, School of MedicineKeio UniversityTokyoJapan

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