Neurochemical Research

, Volume 10, Issue 9, pp 1203–1219 | Cite as

Two-dimensional electrophoresis of cerebrospinal fluid proteins in normal and pathological conditions

  • F. Bracco
  • P. Gallo
  • B. Tavolato
  • L. Battistin
Original Articles


The two-dimensional polyacrylamide gel electrophoresis technique has been adapted for the analysis of human cerebrospinal fluid proteins. Proteins were detected by Coomassie brilliant blue stain and/or by silver stain. Highly reproducible protein patterns were obtained. We analyzed ten normal CSF specimens, thirty pathological CSF specimens and the corresponding sera. We mapped the protein patterns observed by examination of serum/CSF differences and by immunofixation. Preliminary observations on the changes in protein patterns in CSF specimens from patients with neurological disorders are reported.


Polyacrylamide Neurological Disorder Pathological Condition Coomassie Brilliant Blue 
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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • F. Bracco
    • 1
  • P. Gallo
    • 1
  • B. Tavolato
    • 1
  • L. Battistin
    • 1
  1. 1.Institute of NeurologyUniversity of PadovaPadovaItaly

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