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Quantitation of the β-bungarotoxin-induced release of lactate dehydrogenase from cerebrocortical synaptosomes

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Abstract

Treatment with β-bungarotoxin for 1 h induces lactate dehydrogenase release from cerebrocortical synaptosomes. The effect is Ca2+-dependent as suggested by the inhibition observed with EGTA. An inhibition of the effect can be also obtained by addition of Sr2+ ions, suggesting that the phospholipase A2 activity associated to the toxin is involved in the efflux of the enzyme. The β-bungarotoxin-induced release of synaptoplasmic lactate dehydrogenase is a saturable effect, showing a half-maximal effect concentration of 32 nM and a maximal efflux of 25% of the total synaptosomal enzyme as calculated by double-reciprocal plot.

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  1. Departmento de Bioquímica, Biología Molecular y Celular, Universidad de Zaragoza. Facultad de Veterinaria, Miguel Servet, 177, 50013, Zaragoza, Spain

    C. Martinez, A. Pérez-Martos & M. J. López Pérez

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  1. C. Martinez
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  2. A. Pérez-Martos
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  3. M. J. López Pérez
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Martinez, C., Pérez-Martos, A. & López Pérez, M.J. Quantitation of the β-bungarotoxin-induced release of lactate dehydrogenase from cerebrocortical synaptosomes. Neurochem Res 14, 865–869 (1989). https://doi.org/10.1007/BF00964816

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