Abstract
Initial velocities of uptake ofl-glutamic acid and 2-deoxy-d-glucose (2-Dg) have been measured in cortical synaptosomes from rats which had been exposed to oxygen at high pressure (OHP) and compared to similar measurements in normobaric controls. Exposure to OHP had no significant effect on glutamate uptake at any combination of sodium and glutamate used. In contrast, OHP reduced 2-Dg uptake by an average of 17.5%. Although Kt was little affected, OHP exposure reduced apparent maximal transport capacity by 15%. Since hyperbaria with normal pO2 had no significant effect on uptake, the effect of OHP is an oxygen effect, rather than a pressure effect. The effects of OHP on uptake do not parallel the effects of age; glutamate transport capacity was reduced in aged animals, while 2-Dg transport was unaffected.
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Crapo, J. D., andTierney, D. L. 1974. Superoxide dismutase and pulmonary oxygen toxicity. Amer. J. Physiol 226:1401–1407.
Fridovich, I. 1982. Superoxide dismutase in biology and medicine. In: Pathology of Oxygen, Anne P. Autor ed. New York Academic Press.
Gregory, E. M., andFridovich, I. 1973a. Induction of superoxide dismutase by molecular oxygen. J. Bacteriol. 114:543–548.
Gregory, E. M., andFridovich, I. 1973b. Oxygen toxicity and the superoxide dismutase. J. Bacteriol. 114:1193–1197.
Gregory, E. M., Goscin, S. A., andFridovich, I. 1974. Superoxide, dismutase and oxygen toxicity in a eukaryote. J. Bacteriol. 117:456–460.
Harman, D. 1978. Free radical theory of aging: nutritional implication. Age 1:143–150.
Harman, D., Hendricks, S., Eddy, D. E., andSeibold, J. 1976. Free radical theory of aging: effect of dietary fat on central nervous system function. J. Am. Ger. Soc. 24:301–307.
Haugaard, N. 1968. Cellular mechanisms of oxygen toxicity. Physiol. Rev. 48:311–373.
Leibovitz, B. E., andSiegel, B. V. 1980. Aspects of free radical reactions in biological systems: aging. J. Geront. 35:45–56.
Massie, H. R., andWilliams, T. R. 1980. Singlet oxygen and aging in Drosophila. Gerontology 26:16–21.
Nelder, J. A., andMead, R. 1965. A simplex method for function optimization. Comput. J. 7:308–315.
Nohl, H., Hegner, D., andSummer, K. H. 1979. Responses of mitochondrial superoxide dismutase, catalase and glutathione peroxidase activities to aging. Mech. Age Develop. 11:145–151.
Tan, C. T., Tan, K. S., andHsia, J. C. 1978. Spin trapping and spin labeling studies of oxygen toxicity. Undersea Biomed. Res. 5 (Suppl.):23–24.
Wheeler, D. D. 1980. Aging of membrane, transport mechanisms in the central nervous system—high affinity glutamic acid transport in rat cortical synaptosomes. Exp. Geront. 15:269–284.
Wheeler, D. D. 1981. Aging of membrane transport mechanisms in the central nervous system—2-deoxy-d-glucose transport in cortical synaptosomes from the Long-Evans rat. Exp. Geront. 16:247–252.
Wheeler, D. D. 1982. Aging of membrane transport mechanisms in the central nervous system. GABA transport in rat cortical synaptosomes. Exp. Geront. 17:71–85.
Wheeler, D. D. 1983. Aging of the high affinity GABA transporter in synaptosomes from the hypothalamus of the rat. Exp. Geront. 18:125–135.
Wheeler, D. D., andBlackburn, J. G. 1983. Effect of hyperbaric oxygen on GABA transport in rat brain synaptosomes. J. Neural Transmission 57:167–185.
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Wheeler, D.D., Blackburn, J.G. Effect of hyperbaric oxygen on 2-deoxy-d-glucose andl-glutamate transport in rat cortical synaptosomes. Neurochem Res 9, 1075–1083 (1984). https://doi.org/10.1007/BF00964803
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DOI: https://doi.org/10.1007/BF00964803