Advertisement

Neurochemical Research

, Volume 9, Issue 8, pp 1051–1063 | Cite as

RNA synthesis in neuronal and glial cell nuclei from rat cerebral hemispheres during early postnatal development

  • I. Serra
  • R. Avola
  • A. Vanella
  • B. Lombardo
  • A. Agodi
  • A. M. Giuffrida
Original Articles

Abstract

RNA synthesis in rat cerebral hemispheres at 1, 5, and 10 days of age and the relative contribution brought by neuronal and glial nuclei to RNA synthesis was investigated. The experiments were carried out both in vivo (by i.p. injection of [3H]uridine) and in vitro (either by incubation of tissue slices with [3H]uridine or by determination of RNA polymerase activities). The labeling of RNA decreases from 1 to 10 days of age both in vivo and in vitro; the decrease is of the same extent in neuronal and glial nuclei. RNA polymerase activity Mg2+-dependent does not change significantly from 1 to 10 days of age either in total, in neuronal, or in glial nuclei, whereas the Mn2+-dependent activity increases significantly over the same developmental period studied. The significance of RNA polymerase assay as an index of in vivo RNA synthesis is discussed.

Keywords

Glial Cell Cell Nucleus Uridine Cerebral Hemisphere Developmental Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lim, L. 1977. Regulation of RNA metabolism in the developing brain. Pages 15–41,in A. N. Davison (ed.), Biochemical correlates of brain structure and function, Academic Press, London.Google Scholar
  2. 2.
    Orrego, F. 1967. Synthesis of RNA in normal and electrically stimulated brain cortex slices in vitro. J. Neurochem. 14:851–858.Google Scholar
  3. 3.
    Bondy, S. C., andRoberts, S. 1969. Developmental and regional variations in ribonucleic acid synthesis on cerebral chromatin. Biochem. J. 115:341–349.Google Scholar
  4. 4.
    Guroff, G., andBrodsky, M. 1971. Enzymes of nucleic acid metabolism in the brains of young and adult rats. J. Neurochem. 18:2077–2084.Google Scholar
  5. 5.
    Giuffrida, A. M., Serra, I., Vanella, A., andCozzolino, A. 1974. Biosynthesis of nucleic acids in different regions of rat brain during postnatal development. Ital. J. Biochem. 23:87–95.Google Scholar
  6. 6.
    Johnson, T. C. 1967. The effects of maturation on in vitro RNA synthesis by mouse brain cells. J. Neurochem. 14:1075–1081Google Scholar
  7. 7.
    Johnson, T. C. 1968. Cell-free protein synthesis by mouse brain during early development. J. Neurochem. 15:1189–1194.Google Scholar
  8. 8.
    Giuffrida, A. M., Hamberger, A., andSerra, I. 1976. Biosynthesis of DNA and RNA in neuronal and glial cells from various regions of developing rat brain. J. Neurosci. Res. 2:203–215.Google Scholar
  9. 9.
    Gadaleta, M. N., Giuffrida, A. M., Renis, M., Serra, I., Del Prete, G., Geremia, E., andSaccone C. 1979. Macromolecular synthesis in mitochondria isolated from different regions of developing rat brain. Neurochem. Res. 4:25–35.Google Scholar
  10. 10.
    Giuffrida, A. M., Gadaleta, M. N., Serra, I., Renis, M., Geremia, E., Del Prete, G. andSaccone, C. 1979. Mitochondrial DNA, RNA and protein synthesis in different regions of developing rat brain. Neurochem. Res. 4:37–52.Google Scholar
  11. 11.
    Avitabile, M., Serra, I., Mathias, A. P., andGiuffrida, A. M. 1981. Effect of undernutrition on RNA synthesis in various regions of developing rat brain. Bull. Mol. Biol. Med. 6:32–43.Google Scholar
  12. 12.
    Serra, I., Cupello, A., Gadaleta, M. N., Viola, M., Ragonese, P., and 1983. Labeling of RNA in young and adult rat brain: evidence for different RNA processing. Neurochem. Res. 8:433–447.Google Scholar
  13. 13.
    Berthold, W., andLim, L. 1976. The metabolism of high molecular weight ribonucleic acid, including polyadenylated species, in the developing rat brain. Biochem. J. 154:517–527.Google Scholar
  14. 14.
    Berthold, W., andLim, L. 1976. Nucloe-cytoplasmic relationship of high-molecular weight ribonucleic acid, including polyadenylated species in the developing rat brain. Biochem. J. 154:529–539.Google Scholar
  15. 15.
    Chambon, P. 1975. Eucaryotic nuclear RNA polymerases. Ann. Rev. Biochem. 44:613–638.Google Scholar
  16. 16.
    Barondes, S. H. 1964. Studies with an RNA polymerase from brain. J. Neurochem. 11:663–669.Google Scholar
  17. 17.
    Bondy, S. C., andWaelsch, H. 1964. RNA polymerase in the central nervous system. Life Science. 3:633–636.Google Scholar
  18. 18.
    Rappoport, D. A., Fritz, O. R., andYamagami, S. 1971. Development, Pages 439–479,in A. Lajtha (ed.), Handbook of Neurochemistry. Vol. 5, part B, Plenum Press. New York.Google Scholar
  19. 19.
    Gomez, C. J., Garcìa Argiz, C. A., Franzoni, L., andKrawiec, L. 1971. Ribonucleic acid synthesis in isolated cell nuclei of developing rat brain. Neurobiology. 1:129–143.Google Scholar
  20. 20.
    Banks, S. P., andJohnson, T. C. 1973. Developmental alterations in RNA synthesis in isolated mouse brain nucleoli. Biochim. Biophys. Acta. 294:450–460.Google Scholar
  21. 21.
    Banks, S. P., andJohnson, T. C. 1972. Maturation-dependent events related to DNA-dependent RNA synthesis in intact mouse brain nuclei. Brain Res. 41:155–169.Google Scholar
  22. 22.
    Giuffrida, A. M., Cox, D., Mathias, A. P. 1975. RNA polymerase activity in various classes of nuclei from different regions of rat brain during postnatal development. J. Neurochem. 24:749–755.Google Scholar
  23. 23.
    Giuffrida, A. M., Cambria, A., Serra, I., Avitabile, M. 1975. Nucleolar and nucleoplasmic RNA polymerase activity in different regions of rat brain during postnatal development. Ital. J. Biochem. 24:288–307.Google Scholar
  24. 24.
    Banks-Schlegel, S. P., andJohnson, T. C. 1975. RNA metabolism in isolated mouse brain nuclei during early postntal develoment. J. Neurochem. 24:947–952.Google Scholar
  25. 25.
    Greenwood, P. D., andBrown, I. R. 1982. Developmental changes in DNase I digestibility and RNA template activity of neuronal nuclei relative to the postnatal appearance of a short DNA repeat length. Neurochem. Res. 7:965–976.Google Scholar
  26. 26.
    McIlwain, H., andBuddle, H. L. 1953. Techniques in tissue metabolim. 1. A mechanical chopper. Biochem. J. 53:412–420.Google Scholar
  27. 27.
    Thompson, R. J. 1973. Studies on RNA synthesis in two populations of nuclei from the mammalian cerebral cortex. J. Neurochem. 21:19–40.Google Scholar
  28. 28.
    Schmidt, G., andTannhauser, S. J. 1945. A method for the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animals tissue. J. Biol. Chem. 161:83–89.Google Scholar
  29. 29.
    Serra, I., Hamberger, A., Ragonese, P., andGiuffrida, A. M. 1982. Effect of undernutrition on DNA and RNA synthesis in subcellular fractions from different regions of the developing rat brain. Neurochem. Res. 7:887–904.Google Scholar
  30. 30.
    Plagemann, P. G. W. 1971. Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. I. Kinetics of incorporation of nucleosides into nucleotide pools and pool sizes during growth cycle. J. Cell Physiol. 77:213–240.Google Scholar
  31. 31.
    Burton, K. 1956. A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62:315–322.Google Scholar
  32. 32.
    Mandel, P. andWintzerith, M. 1980. Free nucleotides and nucleic acids during brain development. Pages 241–282 in Kuman S. (ed) Biochemistry of Brain, Pergamon Press, London.Google Scholar
  33. 33.
    Kedinger, C., Nuret, P. andChambon, P. 1971. Structural evidence for two α-amanitin sensitive RNA polymerases in calf thymus. Febs Lett. 15:169–174.Google Scholar
  34. 34.
    Flangas, A. L., andBowman R. E. 1970. Differential metabolism of RNA in neuronalenriched and glial-enriched fractions of rat cerebrum. J. Neurochem. 18:1237–1245.Google Scholar
  35. 35.
    Jarlstedt, J., andHamberger, A. 1971. Patterns and labeling characteristics in neuronal and glial RNA. J. Neurochem. 18:921–930.Google Scholar
  36. 36.
    Yanagihara, T. 1979. Protein and RNA syntheses and precursor uptake with isolated nerve and glial cells. J. Neurochem. 32:169–177.Google Scholar
  37. 37.
    Thomas, J. O., andThompson, R. J. 1977. Variation in chromatin structure in two cell types from the same tissue: a short DNA repeat length in cerebral cortex neurons. Cell, 10:633–640.Google Scholar
  38. 38.
    Weck, P. K., andJohnson, T. C. 1978. Nuclear-cytosol interactions that facilitate release of RNA from mouse brain nuclei. J. Neurochem. 30:1057–1065.Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • I. Serra
    • 1
  • R. Avola
    • 1
  • A. Vanella
    • 1
  • B. Lombardo
    • 1
  • A. Agodi
    • 1
  • A. M. Giuffrida
    • 1
  1. 1.Institute of BiochemistryUniversity of CataniaCataniaItaly

Personalised recommendations