Neurochemical Research

, Volume 10, Issue 1, pp 99–106 | Cite as

Association of [14C]glutamate with the protein components of synaptic membrane preparations

  • Leena Turpeenoja
  • Pekka Lähdesmäki
Original Articles


Calf brain synaptic plasma membranes were saturated under extracellular conditions with [14C]glutamic acid and the resulting labelled membranes fractionated with 0.9% NaCl, distilled water,n-butanol-water, 0.05 mol/L NaOH and 0.5% Triton X-100 solutions in this order. Triton X-100 was the most effective solubilizer, liberating altogether about 24% of the membrane proteins, but only 4–7% of the label, while NaOH was the most potent solubilizer for the protein-bound label (50–70%). Slab gel electrophoresis showed significant qualitative differences in the banding patterns of the protein extracts, but only two fractions, a low-molecular weight (around 15 kd) and a high-molecular weight (>200 kd) fraction bound [14C]glutamate.


Glutamate Glutamic Acid Banding Pattern Qualitative Difference Protein Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Leena Turpeenoja
    • 1
  • Pekka Lähdesmäki
    • 1
  1. 1.Department of BiochemistryUniversity of OuluOulu 57Finland

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