Neurochemical Research

, Volume 5, Issue 7, pp 719–730 | Cite as

[3H]Acetylcholine and [3H]5-hydroxytryptamine release from rat midbrain slices and the effects of calcium and phenobarbital

  • Judith A. Richter
  • Susan K. Jackson
Original Articles


The K-stimulated release of [3H]ACh from rat midbrain slices prelabeled by incubation with [3H]choline was dependent on extracellular Ca. Phenobarbital inhibited the K-stimulated [3H]ACh release and the IC50 was equal to that found for K-stimulated endogenous ACh release. These results support the suggestion that barbiturates primarily inhibit the Ca-dependent stimulated release of ACh and affect ACh synthesis only indirectly. K-Stimulated release of [3H]5-HT was also inhibited by removing Ca from the medium or by adding phenobarbital which further supports the effects of barbiturates on the depolarization-induced release process. Fluoxetine, an inhibitor of 5-HT uptake, increased the amount of [3H]5-HT found in the medium but did not fully block the uptake of [3H]5-HT in this slice preparation.


Calcium Choline Acetylcholine Fluoxetine Phenobarbital 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • Judith A. Richter
    • 1
  • Susan K. Jackson
    • 1
  1. 1.Institute of Psychiatric Research and Departments of Pharmacology and PsychiatryIndiana University School of MedicineIndianapolis

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