Neurochemical Research

, Volume 10, Issue 7, pp 871–877 | Cite as

Effect of picrotoxinin on benzodiazepine receptor binding

  • Andrew Y. Chweh
  • Rafael A. Ulloque
  • Ewart A. Swinyard
  • Harold H. Wolf
Original Articles


The effect of picrotoxinin on [3H]flunitrazepam binding to benzodiazepine receptors was investigated. In mouse forebrain membranes, picrotoxinin inhibited basal, GABA- and pentobarbital-stimulated [3H]flunitrazepam binding; this inhibitory activity was temperature- and chloride ion-dependent. Scatchard analysis of the data indicates that picrotoxinin decreases the number of binding sites without alterating binding affinity. In cerebellar membranes, picrotoxinin did not alter [3H]flunitrazepam receptor binding.


Chloride Inhibitory Activity Binding Affinity Receptor Binding Flunitrazepam 
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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Andrew Y. Chweh
    • 1
  • Rafael A. Ulloque
    • 1
  • Ewart A. Swinyard
    • 1
  • Harold H. Wolf
    • 1
  1. 1.Department of Biochemical Pharmacology and Toxicology College of PharmacyUniversity of UtahSalt Lake City

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