Neurochemical Research

, Volume 10, Issue 6, pp 809–817 | Cite as

Further characterization of [3H]fulunitrazepam binding sites on cultured mouse astroglia

  • M. Tardy
  • C. Fages
  • G. Dupre
  • M. F. Costa
  • J. Bardakdjian
  • B. Rolland
Original Articles


Astroglial cells in primary cultures bind [3H]flunitrazepam with a high affinity on a single type of site and on a number of binding sites which increased during astroglial growth and differentiation. These binding sites show a particular pharmacological spectrum characterized by an inhibition of high affinity by RO-5-4864 (4′-chlorodiazepam), an anticonvulsant of the benzodiazepine family and by an inhibition of binding of lower affinities by diazepam clonazepam and clobazam. RO-5-4864 and clonazepam compete for the same binding site in astroglia. The heat stability and the hormonal modulation by thyroxine are similar for astroglia and neuronal-cells. Benxodiazepines modulate the astroglial 5-HT receptor. Such an effect could be a possible physiological response to benzodiazepines for astroglial cells in primary cultures.


Binding Site Diazepam Primary Culture Physiological Response Lower Affinity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • M. Tardy
    • 1
  • C. Fages
    • 1
  • G. Dupre
    • 1
  • M. F. Costa
    • 1
  • J. Bardakdjian
    • 1
  • B. Rolland
    • 1
  1. 1.Department de BiochimieC.H.U. Henri MondorCreteil CedexFrance

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