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Further characterization of [3H]fulunitrazepam binding sites on cultured mouse astroglia

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Abstract

Astroglial cells in primary cultures bind [3H]flunitrazepam with a high affinity on a single type of site and on a number of binding sites which increased during astroglial growth and differentiation. These binding sites show a particular pharmacological spectrum characterized by an inhibition of high affinity by RO-5-4864 (4′-chlorodiazepam), an anticonvulsant of the benzodiazepine family and by an inhibition of binding of lower affinities by diazepam clonazepam and clobazam. RO-5-4864 and clonazepam compete for the same binding site in astroglia. The heat stability and the hormonal modulation by thyroxine are similar for astroglia and neuronal-cells. Benxodiazepines modulate the astroglial 5-HT receptor. Such an effect could be a possible physiological response to benzodiazepines for astroglial cells in primary cultures.

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References

  1. Mohler, H., andOkada, T. 1977. Benzodiazepine receptors: Demonstration in the central nervous system. Science, 18:849–851.

    Google Scholar 

  2. Squires, R., andBraestrup, C. 1977. Benzodiazepine receptors in rat brain. Nature (Lond.), 266:732–734.

    Google Scholar 

  3. Braestrup, C., Abechsten, R., andSquires, R. F. 1977. High densities of benzodiazepine receptors in human cortical areas. Nature (Lond.) 269:702–704.

    Google Scholar 

  4. Syapin, P., andSkolnick, P. 1979. Characterization of benzodiazepine binding sites in cultured cells of neural origin. J. Neurochem. 32:1047–1051.

    PubMed  Google Scholar 

  5. McCarthy, K., andHarden, T. K. 1981. Identification of two benzodiazepine binding sites on cells cultured from rat cerebral cortex. J. Pharmacol. Exp. Ther. 216:183–191.

    PubMed  Google Scholar 

  6. Tardy, M., Costa, M. F., Rolland, B., Fages, C., andGonnard, P. 1981. Benzodiazepine receptors on primary cultures of mouse astrocytes. J. Neurochem. 36:1587–1589.

    PubMed  Google Scholar 

  7. Hertz, L., andMuker, S. 1980. Diazepam receptors on mouse astrocytes in primary cultures; displacement by pharmacologically active concentrations of benzodiazepines or barbiturates. Can. J. Physiol. Pharmacol. 2:217–220.

    Google Scholar 

  8. Kammerman Phyllis Sher, andMachen, V. L. 1984. Properties of [3H]diazepam binding sites on cultured murine glia and neurons. Devt. Brain Res. 14:1–6.

    Google Scholar 

  9. Henn, F. A., andHenke, D. J. 1978. Cellular localization of [3H]diazepam receptors. Neuropharm. 17:985–988.

    Google Scholar 

  10. Hertz, L., andRichardson, J. S. 1984. Is neuropharmacology morely the pharmacology of neurons or are astrocytes important too. TIPS Rev. July 272–276.

  11. Tardy, M., Rolland, B., Bardakdjian, J., andGonnard, P. 1978. Action of β-(4-chlorophenyl) GABA on uptake and metabolism of GABA indifferent subcellular fractions of rat brain. Experientia 34:1137–1138.

    PubMed  Google Scholar 

  12. Clarke, W. R., Jones, L. R., andLefkowelz, R. J. 1978. Hepatic α-adrenergic receptors. Identification and subcellular localization using [3H] dihydroergoryptine. J. Biol. Chem. 253:5975–5979.

    PubMed  Google Scholar 

  13. Bardakdjian, J., Tardy, M., Pimoule, C., andGonnard, P. 1979. GABA metabolism in cultured glial cells. Neurochem. Res. 4:519–529.

    Google Scholar 

  14. Tardy, M., Costa, M. F., Fages, C., Bardakdjian, J., andGonnard, P. 1982. Serotonin uptake and binding by astroglial primary cultures of mouse brain. Develop. Neuroscience, 5:19–26.

    Google Scholar 

  15. Seamon, K. B., Padgett, W., andDaly, J. W. 1981. Forskolin: unique diterpen activator of adenylate cyclase in membranes and in intact cells. Proc. Natl Acad. Sci. (78). 6:3363–3367.

    Google Scholar 

  16. Tardy, M., Fages, C., Rolland, B., Bardakdjian, J., andGonnard, P., 1981. Effect of PGE1 and dBcAMP on the morphology of cells in primary cultures and on enzymes of GABA and Glu Metabolism. Experientia, 37:19–20.

    PubMed  Google Scholar 

  17. Tardy, M., Costa, M. F., Fages, C., Lacombe, C., andRolland, B. 1984. Thyroxin effect on astroglial flunitrazepam binding. Cell. Mol. Biol. 30(5):459–461.

    PubMed  Google Scholar 

  18. Tardy, M., Rolland, B., andCaldani, M. 1983. Astroglial cells are glucocorticoid target cells in brains. J. Ster. Biochem. (abstract) 496, vol. 19 suppl.

  19. Bowling, A. C., andDeLorenzo, R. J. 1982. Micromolar benzodiazepine receptors. Identification and characterization in the central nervous system. Science 216:1247–1250.

    PubMed  Google Scholar 

  20. Hess, S. M., Chasin, M., Free, C. A., andHarris, D. N. 1975. Modulation of cyclic AMP systems. Biochem. Psychopharmacol. 14:153–158.

    Google Scholar 

  21. Fillion, G., Beaudon, D., Roussele, J. C., andJacob J. 1981. 3H-5HT binding sites and 5HT sensitive adenylate cyclase in glial cell membrane fraction. Brain Res. 198:361–374.

    Google Scholar 

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Authors and Affiliations

  1. Department de Biochimie, C.H.U. Henri Mondor, 51, av. de Lattre de Tassigny, 94010, Creteil Cedex, France

    M. Tardy, C. Fages, G. Dupre, M. F. Costa, J. Bardakdjian & B. Rolland

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  1. M. Tardy
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  2. C. Fages
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  3. G. Dupre
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  4. M. F. Costa
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  5. J. Bardakdjian
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  6. B. Rolland
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Tardy, M., Fages, C., Dupre, G. et al. Further characterization of [3H]fulunitrazepam binding sites on cultured mouse astroglia. Neurochem Res 10, 809–817 (1985). https://doi.org/10.1007/BF00964538

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  • DOI: https://doi.org/10.1007/BF00964538

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