Neurochemical Research

, Volume 10, Issue 6, pp 809–817 | Cite as

Further characterization of [3H]fulunitrazepam binding sites on cultured mouse astroglia

  • M. Tardy
  • C. Fages
  • G. Dupre
  • M. F. Costa
  • J. Bardakdjian
  • B. Rolland
Original Articles

Abstract

Astroglial cells in primary cultures bind [3H]flunitrazepam with a high affinity on a single type of site and on a number of binding sites which increased during astroglial growth and differentiation. These binding sites show a particular pharmacological spectrum characterized by an inhibition of high affinity by RO-5-4864 (4′-chlorodiazepam), an anticonvulsant of the benzodiazepine family and by an inhibition of binding of lower affinities by diazepam clonazepam and clobazam. RO-5-4864 and clonazepam compete for the same binding site in astroglia. The heat stability and the hormonal modulation by thyroxine are similar for astroglia and neuronal-cells. Benxodiazepines modulate the astroglial 5-HT receptor. Such an effect could be a possible physiological response to benzodiazepines for astroglial cells in primary cultures.

Keywords

Binding Site Diazepam Primary Culture Physiological Response Lower Affinity 

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • M. Tardy
    • 1
  • C. Fages
    • 1
  • G. Dupre
    • 1
  • M. F. Costa
    • 1
  • J. Bardakdjian
    • 1
  • B. Rolland
    • 1
  1. 1.Department de BiochimieC.H.U. Henri MondorCreteil CedexFrance

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