Neurochemical Research

, Volume 10, Issue 6, pp 789–796 | Cite as

Lack of effect of GABA on [3H]leucine incorporation into a rat oviduct ribosomal system

  • Luis M. Orensanz
  • Carmen Azuara
  • Inmaculada Fernández
Original Articles


A ribosomal system for [3H]leucine incorporation was isolated from the rat oviduct in order to study the possible effect of GABA on [3H]leucine incorporation during the estrous cycle. The system showed an absolute requirement for Mg2+ and about 50% dependence on an energy source. Optimal [3H]leucine incorporation occurred under 3–6 mM Mg2+ and 100 mM K+ and was higher indiestrous-1 than in estrous or proestrous. GABA (10 mM) had no effect on [3H]leucine incorporation in any of the three estrous phase studied.


Energy Source Leucine Estrous Cycle Absolute Requirement Leucine Incorporation 
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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Luis M. Orensanz
    • 1
  • Carmen Azuara
    • 1
  • Inmaculada Fernández
    • 1
    • 2
  1. 1.Departamento de InvestigaciónCentro Ramón y CajalMadridSpain
  2. 2.Departamento de BioquímicaC.U.I. Arcos de JalónMadridSpain

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