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Synthesis of thiamine triphosphate in rat brain in vivo

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Abstract

Thiamine metabolism in vivo was studied by intracerebroventricular injection of labeled thiamine in rat brain. Labeled thiamine was found to be rapidly converted to the phosphorylated thiamine esters. The distribution of the radioactive thiamine compounds was reached to steady state at 3 hr after injection: thiamine, thiamine monophosphate, thiamine pyrophosphate, and thiamine triphosphate were 8–12%, 12–14%, 72–74%, and 2–3%, respectively, in cerebral cortex. The presence of labeled thiamine triphosphate in the brain was further confirmed by the treatment with thiamine triphosphatase which had an absolute substrate specificity for thiamine triphosphate. These results suggest that thiamine triphosphate is synthesized in vivo in rat brain.

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Authors and Affiliations

  1. Department of Pharmacology Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, 565, Suita, Osaka, Japan

    Heitaroh Iwata, Yasunori Yabushita, Tadashi Doi & Toshio Matsuda

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  1. Heitaroh Iwata
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  2. Yasunori Yabushita
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  3. Tadashi Doi
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  4. Toshio Matsuda
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Iwata, H., Yabushita, Y., Doi, T. et al. Synthesis of thiamine triphosphate in rat brain in vivo. Neurochem Res 10, 779–787 (1985). https://doi.org/10.1007/BF00964535

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