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β-Alanine transport into plasma membrane vesicles derived from rat brain synaptosomes

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Abstract

Transport of β-alanine has been demonstrated in membrane vesicles isolated from rat brain, using artificially imposed ion gradients as the sole energy source. The uptake of β-alanine is strictly dependent on the presence of Na+ and Cl− in the medium, and the process can be driven either by an Na+ gradient (out > in) or by a Cl− gradient (out > in) when the other essential ion is present. The process is stimulated by a membrane potential (negative inside) as demonstrated by the effect of ionophore valinomycin and anions with different permeabilities. β-Alanine uptake is inhibited by the presence of GABA.

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular Centro de Biología Molecular Facultad de Ciencias, Universidad Autónoma, Madrid-34, Spain

    Francisco Zafra, M. Carmen Aragon, Fernando Valdivieso & Cecilio Gimenez

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  1. Francisco Zafra
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  2. M. Carmen Aragon
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  3. Fernando Valdivieso
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  4. Cecilio Gimenez
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Zafra, F., Aragon, M.C., Valdivieso, F. et al. β-Alanine transport into plasma membrane vesicles derived from rat brain synaptosomes. Neurochem Res 9, 695–707 (1984). https://doi.org/10.1007/BF00964516

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  • DOI: https://doi.org/10.1007/BF00964516

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