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Is there a “dopaminergic glial cell”?

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Abstract

Intracellular cAMP increased 9-fold in cerebral hemisphere primary cultures after incubation with dopamine (10−4M). The effect was dose- and time-dependent (10−6 M-10−4M; 2–10 minutes). It was mimicked, to some extent, by the partial agonist apomorphine (10−5 M-10−4 M) and antagonized by fluphenazine (10−5 M-10−4 M). The elevation of cAMP caused by dopamine was incompletely antagonized by propanolol (10−5 M-10−4 M), obviating an interaction with β-adrenergic receptors. A β-adrenergic effect was antagonized by propranolol but only slightly by fluphenazine. The effect of dopamine on cAMP-level was more pronounced in a subpopulation of the hemisphere culture, i.e. in astroglial cultures from the striatum, 12-fold compared with controls at 10−4 M. No dopamine stimulated formation of cAMP was found in primary cultures from brain-stem. The results demonstrated some heterogeneity among astroglial cells. The cultures used contained mainly astroglial-like cells, as judged from immnohistochemical localization mainly astroglial-like cells, as judged from immunohistochemical localization of the glial specific proteins S 100 and GFA (α-albumin). No mature neurons or oligodendroglial cells have so far been demonstrated in the cultures.

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Authors and Affiliations

  1. Institute of Neurobiology, University of Göteborg, Sweden

    Elisabeth Hansson & Lars Rönnbäck

  2. Division of Experimental Medicine, National Defence Research Institute, Umeå, Sweden

    Åke Sellström

Authors
  1. Elisabeth Hansson
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  2. Lars Rönnbäck
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  3. Åke Sellström
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Hansson, E., Rönnbäck, L. & Sellström, Å. Is there a “dopaminergic glial cell”?. Neurochem Res 9, 679–689 (1984). https://doi.org/10.1007/BF00964514

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