Neurochemical Research

, Volume 9, Issue 5, pp 595–606 | Cite as

Developmental changes in the activity and substrate specificities of mouse brain monoamine oxidase

  • Yayoi Koide
  • Kiyofumi Kobayashi
Original Articles


Developmental changes in monoamine oxidase (MAO) activity in the mouse brain were investigated with the substrates β-phenylethylamine (PEA), tryptamine, and 5-hydroxytryptamine (5-HT). In the newborn brain, MAO activity towards PEA was found to be much lower than the adult and to be inhibited by clorgyline in a double-sigmoidal fashion. The inhibition curve shifted to a single-sigmoidal pattern with age. MAO activity towards 5-HT as substrate was inhibited by 90% and in a single-sigmoidal manner by clorgyline throughout the postnatal life. Lineweaver-Burk plots with PEA as substrate presented two linear lines (apparentKm: 28.6 and 4.1 μM) for the newborn and one line (apparentKm: 11.4 μM) for the adult, respectively. The plot with highKm value for the newborn brain disappeared in a clorgyline-treated preparation. These findings suggest that age-dependent alterations in the ratio of MAO-A/MAO-B activity affect the substrate specificity of the enzyme.


Substrate Specificity Monoamine Mouse Brain Monoamine Oxidase Developmental Change 
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Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Yayoi Koide
    • 1
  • Kiyofumi Kobayashi
    • 1
  1. 1.Department of Clinical Neurochemistry Institute for NeurobiologyOkayama University Medical SchoolOkayamaJapan

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