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Neurochemical Research

, Volume 4, Issue 6, pp 731–746 | Cite as

Biochemical characterization of [3H]norepinephrine uptake in dissociated brain cell cultures from chick embryos

  • Douglas W. Hoffman
  • Antonia Vernadakis
Original Articles

Abstract

The uptake of [3H]norepinephrine ([3H]NE) was studied in dissociated brain cell cultures prepared from 8-day-old chick embryos using the whole brain (minus optic lobes). Uptake of [3H]NE, 5×10−9 M, 10 min incubation, in freshly dissociated noncultured embryonic chick brain cells, was detected in 6-day-old embryos; it was temperature and drug (cocaine, metanephrine) sensitive and increased with brain development. In cultured cells, which were assayed at various days in culture, the increase in [3H]NE accumulation per culture was less than that seen in freshly dissociated noncultured embryonic cells. When [3H]NE uptake was expressed per mg protein, a decrease with days in culture was observed, reflecting perhaps a dilution of growth or proliferation of cells not accumulating NE. Metanephrine, 5×10−6 M, an inhibitor of extraneuronal uptake, inhibited [3H]NE in 5-day-old cultures whereas desmethylimipramine, an inhibitor of neuronal uptake, inhibited [3H]NE uptake in 15- and 20-day-old cultures. Cocaine, another neuronal inhibitor, inhibited [3H]NE at 10 and 15 days only. We interpret these findings to suggest that during early growth in culture most neuroblasts accumulate NE nonspecifically and, as neuronal maturation proceeds, NE accumulation becomes specific.

Keywords

Cocaine Norepinephrine Chick Embryo Biochemical Characterization Embryonic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • Douglas W. Hoffman
    • 1
  • Antonia Vernadakis
    • 1
  1. 1.Department of Pharmacology and PsychiatryUniversity of Colorado School of MedicineDenver

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