Abstract
The release of previously accumulated [3H]taurine and [14C]GABA from crude synaptosomal (P2) fractions isolated from rat cerebral cortex was studied using a superfusion system. The spontaneous efflux of [3H]taurine and [14C]GABA was stimulated by elevated concentrations of K+ (15–133 mM) in a concentration-dependent manner. This K+-stimulated release of [14C]GABA but not of [3H]taurine was enhanced in the presence of Ca2+. However, addition of 3 mM Ca2+ to the superfusion medium in the presence of the ionophore A 23187 resulted in a stimulation of the release of both [3H]taurine and [14C]GABA. These results are discussed in connection with the cellular localization of tourine in the central nervous system.
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Placheta, P., Singer, E., Sieghart, W. et al. Properties of [3H]taurine release from crude synaptosomal fractions of rat cerebral cortex. Neurochem Res 4, 703–712 (1979). https://doi.org/10.1007/BF00964467
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DOI: https://doi.org/10.1007/BF00964467