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Neurochemical Research

, Volume 4, Issue 5, pp 627–631 | Cite as

Effect of met-enkephalin and (d-Met2,Pro5)-enkephalinamide on the adenylate cyclase activity of rat brain

  • M. Wollemann
  • A. Szebeni
  • S. Bajusz
  • L. Gráf
Original Articles

Abstract

Among opiatelike peptides, stimulatory as well as inhibitory effects are encountered on adenylate cyclase activity. These actions are dependent not only on the investigated brain region but also on the applied peptide. Met-enkephalin stimulates adenylate cyclase activity in the rat brain stem (d-Met2, Pro5)-enkephalinamide and β-endorphin inhibited it, whereas all three peptides inhibited the activity of cortex. Naloxone antagonized the effects of the applied peptides in the presence of sodium chloride.

Keywords

Sodium Peptide Chloride Sodium Chloride Brain Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Collier, H. O. J., andRoy, A. C. 1974. Morphine-like drugs inhibit the stimulation by E prostaglandins of cyclic AMP formation by rat brain homogenate.Nature (London) 248:24–27.Google Scholar
  2. 2.
    Puri, S. K., Cochin, J., andVolicer, L. 1975. Effect of morphine sulfate on adenylate cyclase and phosphodiesterase activities in rat corpus striatum.Life Sci. 16:759–768.Google Scholar
  3. 3.
    Minneman, K. P., andIversen, L. L. 1976. Enkephalin and opiate narcotics increase cyclic GMP accumulation in slices of rat neostriatum.Nature (London) 262:313–314.Google Scholar
  4. 4.
    Sharma, S. K., Klee, W. A., andNirenberg, M. 1977. Opiate-dependent modulation of adenylate cyclase.Proc. Natl. Acad. Sci. USA 74:3365–3369.Google Scholar
  5. 5.
    Goldstein, A., Cox, B. M., Klee, W. A., andNirenberg, M. 1977. Endorphin from pituitary inhibits cyclic AMP formation in homogenates of neuroblastomaxglioma hybrid cells.Nature (London) 265:362–363.Google Scholar
  6. 6.
    Wollemann, M., Borbola, J., Papp, J. Gy., andSzekeres, L. 1975. Cardiac adenylate cyclase activity in relation to beta-adrenergic responses.J. Mol. Cell. Card. 7:523–533.Google Scholar
  7. 7.
    Lowry, O. H., Rosebrough, H. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.Google Scholar
  8. 8.
    Gráf, L., Barát, E., andPatthy, A. 1976. Isolation of a COOH-terminal β-lipotropin fragment (residues 61–91) with morphine-like analgesic activity from porcine pituitary glands.Acta Biochim. Biophys. Acad. Sci. Hung. 11:121–122.Google Scholar
  9. 9.
    Bajusz, S., Rónai, A. Z., Székely, J. I., Gráf, L., Dunai-Kovács, Zs., andBerzétei, I. 1977. A superactive antinociceptive pentapeptide, (d-Met2,Pro5)-enkephalinamide.FEBS Lett. 76:91–92.Google Scholar
  10. 10.
    Simon, E. J., andHiller, J. M. 1978.In vitro studies on opiate receptors and their ligands.Fed. Proc. 37:141–146.Google Scholar
  11. 11.
    Rossier, J., Vargo, T. M., Minick, S., Ling, N., Bloom, F. E., andGuillemin, R. 1977. Regional dissociation of β-endorphin and enkephalin contents in rat brain and pituitary.Proc. Natl. Acad. Sci. USA 74:5162–5165.Google Scholar
  12. 12.
    Watson, S. J., Richard, C. W., andBarchas, J. D. 1978. Adrenocorticotropin in rat brain: Immunocytochemical localization in cells and axons.Science 200:1180–1182.Google Scholar
  13. 13.
    Lord, J. A. H., Waterfield, A. A., Hughes, J., andKosterlitz, H. W. 1977. Endogenous opioid peptides: Multiple agonists and receptors.Nature (London) 267:495–499.Google Scholar
  14. 14.
    Rónai, A. Z., Gráf, L., Székely, J. I., Dunai-Kovács, Zs., andBajusz, S. 1977. Differential behaviour of LPH-(61-91)-peptide in different model systems: Comparison of the opioid activities of LPH-(61-91)-peptide and its fragments.FEBS Lett. 74:182–184.Google Scholar
  15. 15.
    Calderini, G., Consolazione, A., Garattini, S., andAlgeri, S. 1978. Different effects of methionine-enkephalin and (d-Ala2) methionine-enkephalin amide on the metabolism of dopamine and norepinephrine in rat brain: Fact or artifact?Brain Res. 146:392–399.Google Scholar
  16. 16.
    Székely, J. I., Dunai-Kovács, Zs., Miglécz, E., Rónai, A. Z., andBajusz, S. 1979.In vivo antagonism by naloxone of morphine, β-endorphine and a synthetic enkephalin analog.J. Pharmacol. Exp. Ther. (in press).Google Scholar

Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • M. Wollemann
    • 1
    • 2
  • A. Szebeni
    • 1
    • 2
  • S. Bajusz
    • 1
    • 2
  • L. Gráf
    • 1
    • 2
  1. 1.Institute of BiochemistryBiological Center Hungarian Academy of SciencesSzegedHungary
  2. 2.Institute for Drug ResearchBudapestHungary

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