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Neurochemical Research

, Volume 10, Issue 5, pp 667–675 | Cite as

Transport of monoamine and amino acid neurotransmitters by primary astroglial cultures

  • Elisabeth Hansson
Original Articles

Abstract

The transport of [3H]l-glutamate, [3H]l-aspartate, [3H]γ-aminobutyric acid ([3H]GABA), [3H]dopamine, [3H]norepinephrine and [3H]5-hydroxytryptamine (3H-5-HT) was measured in primary astroglial cultures from newborn rat cerebral hemispheres. There was a high-affinity uptake with aKm of 69.0 μM for L-glutamate, 12.3 μM forl-aspartate and 3.1 μM for GABA. The uptake showed properties of high capacity with aVmax of 17.0 nmol·mg prot−1·min−1 forl-glutamate, 1.1 nmol·mg prot−1·min−1 forl-aspartate and 0.04 nmol·mg prot−1·min−1 for GABA. No high-affinity high capacity transport system was found for the monoamines studies. Autoradiographic examination demonstrated a heavy deposit of grains suggesting a prominent accumulation of [3H]l-glutamate and [3H]l-aspartate in the astroglial-like cells of the cultures, while the [3H]GABA accumulation was less intense. On the other hand, there was only a weak accumulation of grains after incubating the cultures with [3H]dopamine, [3H]norepinephrine or [3H]5-HT. Thus, astroglial cells in culture accumulate amino acid neurotransmitters and monoamines in different ways with a high-affinity high-capacity uptake of glutamate, aspartate and GABA and a diffusion-uptake of dopamine, norepinephrine and 5-HT.

Keywords

Dopamine Norepinephrine Monoamine Astroglial Cell Amino Acid Neurotransmitter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Elisabeth Hansson
    • 1
  1. 1.Institute of NeurobiologyUniversity of GöteborgGöteborgSweden

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