Neurochemical Research

, Volume 9, Issue 4, pp 535–544 | Cite as

Effect of taurine on a benzodiazepine-GABA-chloride ionophore receptor complex in rat brain membranes

  • Heitaroh Iwata
  • Keisuke Nakayama
  • Toshio Matsuda
  • Akemichi Baba
Original Articles

Abstract

Taurine at 10 mM had no effect on basal binding of [3H]diazepam to the membranes, while it significantly inhibited a GABA-stimulated binding of [3H]diazepam in cerebral cortex, hippocampus, but not in cerebellum. The inhibition by taurine in the presence of GABA (1μM to 1 mM) was not competitive. At low concentrations (0.04 to 0.2 nM) the binding of [3H]propyl-β-carboline-3-carboxylate, a ligand exhibiting higher affinity for type I than type II benzodiazepine receptors, was not enhanced by GABA, while the binding of higher concentrations (0.5 nM) was. This GABA enhancement of [3H]propyl-β-carboline-3-carboxylate binding was also selectively blocked by taurine. Pentobarbital increased the binding of [3H]diazepam in a medium containing chloride and this effect was potentiated by taurine at 1–10 mM. These findings may be relevant to the modulatory role of taurine in the central nervous system.

Keywords

Chloride Nervous System Central Nervous System Diazepam Cerebral Cortex 

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Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Heitaroh Iwata
    • 1
  • Keisuke Nakayama
    • 1
  • Toshio Matsuda
    • 1
  • Akemichi Baba
    • 1
  1. 1.Department of Pharmacology Faculty of Pharmaceutical SciencesOsaka UniversitySuita OsakaJapan

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