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Neurochemical Research

, Volume 6, Issue 12, pp 1337–1343 | Cite as

Factors influencing the growth and respiration of rat cerebral astrocytes in primary culture

  • James E. Olson
  • David Holtzman
Comment

Abstract

Cell densities and respiratory rates of astrocytes from neonatal rat brain grown in primary culture were determined after 20–30 days in vitro. Cells grown in flasks reached lower densities (μg DNA/cm2) and higher protein: DNA ratios than cells grown in petri dishes. Respiratory rates were lower for cells grown in flasks compared to cells grown in dishes. The pH of the medium in flasks fell below 6.9 between feedings while the pH of the medium in dishes remained at about 7.2. Cells grown in dishes with the medium pH adjusted to 6.8 also showed lower final cell densities, higher protein: DNA ratios, and lower respiratory rates, compared to cells grown under similar conditions at pH 7.5. Intermediate values of each parameter were found in cells grown at pH 7.5 for one week and then at 6.8 for 20 days. We conclude that the effects of ambient pH account for the differences in growth characteristics and respiratory rates of astrocytes grown in dishes versus those grown in flasks.

Keywords

Cell Density Petri Dish Respiratory Rate Similar Condition Primary Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • James E. Olson
    • 1
  • David Holtzman
    • 1
  1. 1.Departments of Neurology and PediatricsStanford University School of MedicineStanford

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