Neurochemical Research

, Volume 6, Issue 12, pp 1337–1343 | Cite as

Factors influencing the growth and respiration of rat cerebral astrocytes in primary culture

  • James E. Olson
  • David Holtzman


Cell densities and respiratory rates of astrocytes from neonatal rat brain grown in primary culture were determined after 20–30 days in vitro. Cells grown in flasks reached lower densities (μg DNA/cm2) and higher protein: DNA ratios than cells grown in petri dishes. Respiratory rates were lower for cells grown in flasks compared to cells grown in dishes. The pH of the medium in flasks fell below 6.9 between feedings while the pH of the medium in dishes remained at about 7.2. Cells grown in dishes with the medium pH adjusted to 6.8 also showed lower final cell densities, higher protein: DNA ratios, and lower respiratory rates, compared to cells grown under similar conditions at pH 7.5. Intermediate values of each parameter were found in cells grown at pH 7.5 for one week and then at 6.8 for 20 days. We conclude that the effects of ambient pH account for the differences in growth characteristics and respiratory rates of astrocytes grown in dishes versus those grown in flasks.


Cell Density Petri Dish Respiratory Rate Similar Condition Primary Culture 
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  1. 1.
    Kimelberg, H. K., Narumi, S. andBourke R. S. 1978. Enzymatic and morphological properties of primary rat brain astrocyte cultures, and enzyme development in vivo. Brain Res. 153:55–77.PubMedGoogle Scholar
  2. 2.
    Nissen, C., andSchousboe, A. 1979. Activity and isoenzyme pattern of lactate dehydrogenase in astroblasts cultured from brains of newborn mice. J. Neurochem. 32:1787–1792.PubMedGoogle Scholar
  3. 3.
    Hertz, L. 1978. Energy metabolism of glial cells. Pages 121– Schoffeniels, E. Franck, G. Hertz, L. andTower D. B. (eds.), Dynamic Properties of Glia Cells. Pergamon Press, New York.Google Scholar
  4. 4.
    Danes, B. S., andPaul, J. 1961. Environmental factors influencing respiration of strain L cells. Exptl. Cell Res. 24:344–355.PubMedGoogle Scholar
  5. 5.
    Fischer, A. 1921. Growth of fibroblasts and hydrogen ion concentration of the medium. J. Exptl. Med. 34:447–454.Google Scholar
  6. 6.
    Olson, J. E., andHoltzman, D. 1980. Respiration in rat cerebral astrocytes from primary culture. J. Neurosci Res., 5(6):497.PubMedGoogle Scholar
  7. 7.
    Richards, G. M. 1974. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal. Biochem. 57:369–376.PubMedGoogle Scholar
  8. 8.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.PubMedGoogle Scholar
  9. 9.
    Rubin, H. 1971. pH and population density in the regulation of animal cell multiplication. J. Cell Biol. 51:636–702.PubMedGoogle Scholar
  10. 10.
    Hertz L., Bock, E. andSchousboe, A. 1978. GFA content, glutamate uptake and activity of glutamate metabolizing enzymes in differentiating mouse astrocytes in primary cultures. Dev. Neurosci. 1:226–238.Google Scholar
  11. 11.
    Dittman, L., Sensenbrenner, M. Hertz, L. andMandel, P. 1973. Respiration by cultivated astrocytes and neurons from the cerebral hemispheres. J. Neurochem. 21:191–198.PubMedGoogle Scholar
  12. 12.
    Roth-Schechter, B., Sensenbrenner, M. M. andMandel, P. 1976. Effet du pentobarbital sur la morphologie et la respiration des cellules gliales en culture primaire. C. R. Acad. Sci. 283:1333–1335.Google Scholar
  13. 13.
    Kuchler, R. J., andMerchant, D. J. 1958. Growth of tissue cells in suspension. Mich. U. Med. Bull. 24:200–212.Google Scholar

Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • James E. Olson
    • 1
  • David Holtzman
    • 1
  1. 1.Departments of Neurology and PediatricsStanford University School of MedicineStanford

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