Abstract
Rabbit synaptosomes have been used to study the effect of the base-exchange reaction in membrane phospholipids on γ-aminobutyric acid (GABA) transport in vitro. The uptake of GABA was measured after a base-exchange reaction with ethanolamine, choline, orl-serine and after subsequent displacement of these exchanged moieties from lipid by bases of similar or different structures which were added to the synaptosomal medium. Serine incorporation stimulated GABA transport, but its displacement from membrane lipid by choline or ethanolamine induced an inhibition of GABA transport. Ethanolamine incorporation inhibited GABA transport, but its displacement by serine or choline resulted in stimulation of GABA uptake. Choline incorporation also inhibited GABA transport, although less than ethanolamine. The pool size of synaptosomal phospholipids, presumably involved in GABA uptake, accounted for 0.2 to 10% of the total content of membrane phospholipid. Thus, alteration of phospholipid compositior by exchange of the lipid hydrophilic head-groups influences the extent GABA uptake into rabbit synaptosomes.
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Goracci, G., Blomstrand, C., Arienti, G., Hamberger, A., andPorcellati, G. 1973. Base-exchange enzymic system for the synthesis of phospholipids in neuronal and glial cells, and their subfractions: A possible marker for neuronal membranes. J. Neurochem. 20:1167–1180.
De Medio, G. E., Hamberger, A., Sellström, Å., andPorcellati, G. 1977. The phospholipid base-exchange system as a possible modulator of γ-aminobutyric acid transport in brain cells. Neurochem. Res. 2:469–484.
Robertson, R. N., andThompson, T. E. 1977. The function of phospholipid polar groups in membranes. FEBS Lett. 76:16–19.
De Medio, G. E., Trovarelli, G., andPorcellati, G. 1977. Relationship between base-exchange reaction and synaptosomal phospholipid pool in the rabbit brain in vitro. It. J. Biochem. 26:202–214.
De Medio. G. E., Trovarelli, G. Hamberger, A., andPorcellati, G. 1976. Baseexchange pool and GABA uptake in isolated nervous cells. 2nd Congress of Ital. Biochem. Soc., Venice, October 1–4, 1976, Commun. No. 170.
Gaiti, A., Brunetti, M., Woelk, H., andPorcellati, G. 1976. Relationship between base-exchange reaction and microsomal phospholipid pool in the rat brain in vitro. Lipids 11:823–829.
Cotman, C. W., andMatthews, D. A. 1971. Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization. Biochim. Biophys. Acta 249:380–394.
Binaglia, L., Goracci, G., Porcellati, G., Roberti, R., andWoelk, H. 1973. The synthesis of choline and ethanolamine phosphoglycerides in neuronal and glial cells of rabbit in vitro. J. Neurochem. 21:1067–1082.
Gaiti, A., Brunetti, M., andPorcellati, G. 1975. The relationship between the phospholipid pool and the base-exchange in the Ca++-stimulated incorporation of ethanolamine into brain microsomal phospholipids. FEBS Lett. 49:361–364.
Porcellati, G., Arienti, G., Pirotta, M., andGiorgini, D. 1971. Base-exchange reactions for the synthesis of phospholipids in nervous tissue: The incorporation of serine and ethanolamine into the phospholipids of isolated brain microsomes. J. Neurochem. 18:1395–1417.
Gaiti, A., Goracci, G., De Medio, G. E., andPorcellati, G. 1972. Enzymic synthesis of plasmalogens and o-alkyl glycerolipid by base-exchange reaction in the rat brain. FEBS Lett. 27:116–121.
Sellström, Å., andHamberger, A. 1975. Neuronal and glial cells system for γ-aminobutyric acid transport. J. Neurochem. 24:847–852.
Hamberger, A., andSvennerholm, L. 1971. Composition of gangliosides and phospholipids of neuronal and glial cell-enriched fractions. J. Neurochem. 18:1821–1829.
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De Medio, G.E., Trovarelli, G., Hamberger, A. et al. Synaptosomal phospholipid pool in rabbit brain and its effect on GABA uptake. Neurochem Res 5, 171–179 (1980). https://doi.org/10.1007/BF00964330
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DOI: https://doi.org/10.1007/BF00964330