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A novel method for the isolation and study of a magnetotactic bacterium

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Abstract

The magnetococcus, a magnetotactic bacterium, has been grown in a complex simulated natural environment. Sufficiently pure samples of cells were obtained magnetically making axenic cultures unnecessary for many purposes. The magnetococcus is a Gram-negative coccus, 1.6 μm in diameter and readily distinguished by highly refractile inclusions and its magnetotactic behavior. This organism is actively motile by means of two bundles of flagella. Electron dense ferromagnetic inclusions were localized between the flagellar bundles. Collections of magnetococci were morphologically homogeneous and negligibly contaminated by extraneous bacteria. DNA extracted from pooled collections of cells was homogeneous by analytical CsCl centrifugation. The guanine-cytosine content was 61.7%. Total iron by percent cellular dry weight was 3.8%. Comparisons with a previously described magnetotactic marine coccus were made.

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Abbreviations

Tris:

Tris (hydroxymethyl) aminomethane buffer

EDTA:

Dipotassium ethylenediamine tetraacetic acid

GC:

Guanosine cytosine

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Authors and Affiliations

  1. Microbiology Program, Department of Biology, Indiana University, 47401, Bloomington, IN, USA

    Thomas T. Moench & W. A. Konetzka

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  1. Thomas T. Moench
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  2. W. A. Konetzka
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Moench, T.T., Konetzka, W.A. A novel method for the isolation and study of a magnetotactic bacterium. Arch. Microbiol. 119, 203–212 (1978). https://doi.org/10.1007/BF00964274

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  • DOI: https://doi.org/10.1007/BF00964274

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