Abstract
Individual rates of synthesis were determined for the major classes of RNA throughout the yeast-tohyphae transition ofMucor racemosus. Cells at stages throughout development were pulse-labelled with3H-uracil or32P-phosphoric acid, broken under liquid nitrogen and extracted with phenol to isolate the RNA. The major classes of RNA (tRNA, rRNA, precursor rRNA) were separated and quantified on sucrose density gradients. Polyadenylated RNA was identified and quantitated after passage through an oligodeoxythymidylic acid-cellulose column. All species of RNA showed an increased rate of synthesis immediately after the atmospheric shift (CO2-to-air) used to initiate development. All rates accelerated until maximums were observed at the time of hyphal germ tube emergence. Thereafter, during hyphal elongation, the rates decelerated. During the period of most rapid synthesis the ribosomal forms of RNA showed a proportionally higher increase in rate of synthesis than the other forms. Newly synthesized mRNA remained a constant fraction of the total RNA made at all time throughout the morphogenesis.
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Abbreviations
- TMK:
-
tris, magnesium, potassium chloride buffer
- TNE:
-
tris, sodium chloride EDTA buffer
- ANE:
-
acetate, sodium chloride, EDTA buffer
- dT:
-
deoxythymidylic acid
References
Bartnicki-Garcia, S., Nickerson, W. J.: Induction of yeastlike development inMucor by carbon dioxide. J. Bacteriol.84, 829–840 (1962)
Boguslawski, G., Schlessinger, D., Medoff, G., Kobayashi, G.: Ribonucleic acid polymerases of the yeast phase ofHistoplasma capsulatum. J. Bacteriol.118, 480–485 (1974)
Boguslawski, G., Kobayashi, G. S., Schlessinger, D., Medoff, G.: Characterization of an inhibitor of ribonucleic acid polymerase from the mycelial phase ofHistoplasma capsulatum. J. Bactiol.122, 532–537 (1975)
Cheung, S. C., Kobayashi, G. S., Schlessinger, D., Medoff, G.: RNA metabolism during morphogenesis inHistoplasma capsulatum. J. Gen. Microbiol.82, 301–307 (1974)
Ehrenfeld, E. K., Koch, A. L.: RNA synthesis in penicillin spheroplasts ofEscherichia coli. Biochim. Biophys. Acta169, 44–57 (1968)
Hynes, N. E., Phillips S. L.: Turnover of polyadenylate-containing ribonucleic acid inSaccharomyces cerevisiae. J. Bacteriol.125, 595–600 (1976)
Kuo, S. C., Cano, F. R., Lampen, J. O.: Lomofungin, an inhibitor of ribonucleic acid synthesis in yeast protoplasts: its effect on enzyme formation. Antimicrob. Agents Chemother3, 716–722 (1973)
Larsen, A. D., Sypherd, P. S.: Cyclic adenosine 3′,5′-monophosphate and morphogenesis inMucor racemosus. J. Bacteriol.117, 432–438 (1974)
Lawther, R. P., Cooper, T. G.: Kinetics of induced and repressed enzyme synthesis inSaccharomyces cerevisiae. J. Bacteriol.121. 1064–1073 (1975)
Lovett, J. S., Haselby, J. A.: Molecular weights of the ribosomal ribonucleic acid of fungi. Arch. Mikrobiol.80, 191–204 (1971)
Miller, R. V., Sypherd, P. S.: Chemical and enzymatic modification of proteins in the 30S ribosome ofEscherichia coli. J. Molec. Biol.78, 527–538 (1973)
Norris, T. E., Koch, A. L.: Effect of growth rate on the relative rates of synthesis of messenger, ribosomal and transfer RNA inEscherichia coli. J. Molec. Biol.64, 633–649 (1972)
Orlowski, M., Sypherd, P. S.: Protein synthesis during morphogenesis ofMucor racemosus. J. Bacteriol.132, 209–218 (1977)
Orlowski, M., Sypherd, P. S.: Regulation of translation rate during morphogenesis in the fungusMucor. Biochemistry17, 569–575 (1978)
Pearson, J. A., Thomas, K.: Ribosomal RNA synthesis in the mycelium ofPhycomyces blakesleeanus. High molecular weight RNA. Biochim. Biophys. Acta447, 28–35 (1976)
Surdin-Kerjan, Y., de Robichon-Szulmajster, H.: Exitence of two levels of repression in the biosynthesis of methionine inSaccharomyces cerevisiae: effect of lomofungin on enzyme synthesis. J. Bacteriol.122, 367–374 (1975)
Udem, S. A., Warner, J. R.: Ribosomal RNA synthesis inSaccharomyces cerevisiae. J. Molec. Biol.65, 227–242 (1972)
Van Assche, J. A., Carlier, A. R.: The pattern of protein and nucleic acid synthesis in germinating spores ofPhycomyces blakesleeanus. Arch. Mikrobiol.93, 129–136 (1973)
Waldron, C.: Synthesis of ribosomal and transfer ribonucleic acids in yeast during a nutritional shift-up. J. Gen. Microbiol.98, 215–221 (1977)
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Orlowski, M., Sypherd, P.S. RNA synthesis during morphogenesis of the fungusMucor racemosus . Arch. Microbiol. 119, 145–152 (1978). https://doi.org/10.1007/BF00964265
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DOI: https://doi.org/10.1007/BF00964265