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Post-translational changes of chromosomal proteins in rat cerebellum during postnatal development

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Abstract

Acetylation, phosphorylation and methylation of nuclear proteins in rat cerebellum at 10 and 30 days of age were investigated in vitro. Isolated nuclei were incubated in the presence of [1-14C]acetyl CoA, S-adenosyl [methyl-3H]methionine and [γ-32P]ATP and then separated into histones and non histone proteins (NHP), which were further fractionated by polyacrylamide gel electrophoresis. The results obtained indicate that acetylation, phosphorylation and methylation of both basic and acidic proteins decrease from 10 to 30 days of age. Electrophoretic analysis of histones shows that the decrease mainly concerns H1, H3, and H2b fractions. The H3 fraction is always more labeled than the other fractions and shows the major changes during postnatal development. Phosphorylation of H2a and H4 fractions increases from 10 to 30 days of age, whereas acetylation and methylation of these fractions do not show significant changes from 10 to 30 days. The densitometric and radioactive patterns of NHP show considerable changes between 10 and 30 days, especially in the high molecular weight region. The incorporation of14C-acetyl and3H-methyl groups and of32P phosphate appears to be generalized throughout the molecular weight range and decreases from 10 to 30 days of age. The methylation of an as yet unidentified protein with a molecular weight of approximately 110,000 daltons occurred at both ages.

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References

  1. Elgin, S. C. R., andWeintraub, H. 1975. Chromosomal proteins and chromatin structure. Ann. Rev. Biochem. 44:725–774.

    Google Scholar 

  2. Kamiyama, M., andWang, T. Y. 1971. Activated transcription from rat liver chromatin by non-histone proteins. Biochim. Biophys. Acta 228:563–576.

    Google Scholar 

  3. Kamiyama, M., Dastugue, B., andKruh, J. 1971. Action of phosphoproteins and protein kinase from rat liver chromatin on RNA synthesis. Biochem. Biophys. Res. Commun. 44:1345–1350.

    Google Scholar 

  4. Kamiyama, M., Dastugue, B., Defer, N., andKruh, J. 1972. Liver chromatin nonhistone proteins. Partial fractionation and mechanism of action on RNA synthesis. Biochim. Biophys. Acta 277:576–583.

    Google Scholar 

  5. Crepin, M., andDastugue, B. 1979. Regulation of transcription by DNA-bound nonhistone nuclear proteins. Eur. J. Biochem. 99:499–505.

    Google Scholar 

  6. Brown, R., andGreenwood, P. 1982. Chromosomal components in brain cells. Pages 41–69,in Brown I. R. (ed.), Molecular approaches to Neurobiology.

  7. Stein, G. S., Spelsberg, T. C., andKleinsmith, L. J. 1974. Nonhistone chromosomal proteins and gene regulation. Science 183:301–304.

    Google Scholar 

  8. Marushige, K. 1976. Activation of chromatin by acetylation of histone side chains. Proc. Natl. Acad. Sci. USA 73:3937–3941.

    Google Scholar 

  9. Kleinsmith, L. J. 1975. Phosphorylation of non-histone proteins in the regulation of chromosome structure and function. J. Cell Physiol. 85:459–475.

    Google Scholar 

  10. Serra, I., Kamiyama, M., Hashim, G. A., Ragonese, P., andGiuffrida, A. M. 1983. Post-translational modifications of nuclear proteins in rat cerebral hemispheres during postnatal development. J. Neurochem. 40:745–751.

    Google Scholar 

  11. Giuffrida, A. M., Hamberger, A., andSerra, I. 1976. Biosynthesis of DNA and RNA in neuronal and glial cells from various regions of developing rat brain. J. Neurosci. Res. 2:203–215.

    Google Scholar 

  12. Giuffrida, A. M., Gadaleta, M. N., Serra, I., Renis, M., Geremia, E., Del Prete, G., andSaccone, C. 1979. Mitochondrial DNA, RNA and protein synthesis in different regions of developing rat brain. Neurochem. Res. 4:37–52.

    Google Scholar 

  13. Giuffrida, A. M., Cox, D., andMathias, A. P. 1975. RNA polymerase activity in various classes of nuclei from different regions of rat brain during postnatal development. J. Neurochem. 24:749–755.

    Google Scholar 

  14. Balhorn, R., Weston, S., Mazrimas, J. A., andYoung, T. 1978. Endogenous nuclease and chromatin protease activities in rodent tissues. J. Cell Biol. 79, CH669.

    Google Scholar 

  15. Murray, K. 1966. The acid extraction of histones from calf thymus deoxyribonucleoprotein. J. Mol. Biol. 15:409–419.

    Google Scholar 

  16. Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  17. Elgin, S. C. R., andBonner, J. 1970. Limited heterogeneity of the major nonhistone chromosomal proteins. Biochemistry 9:4440–4447.

    Google Scholar 

  18. Balhorn, R., andChalkley, R. 1975. Methods for the assessment of selective histone phosphorylation. Methods in Enzymol. 40:138–144.

    Google Scholar 

  19. Ruiz-Carrillo, A., Wangh, J., andAllfrey, V. G. 1975. Processing of newly synthesized histone molecules. Nascent histone H4 chains are reversibly phpsphorylated and acetylated. Science 190:117–128.

    Google Scholar 

  20. Davie, J. R., andCandido, E. P. M. 1978. Acetylated histone H4 is preferentially associated with template-active chromatin. Proc. Natl. Acad. Sci. USA 75:3574–3577.

    Google Scholar 

  21. Allfrey, V. G., Faulkner, R., andMirsky, A. E. 1964. Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc. Natl. Acad. Sci. USA 51:786–794.

    Google Scholar 

  22. Sarkander, H. J., andKnoll-Kohler, E. 1978. Changing patterns of histone acetylation and RNA synthesis of the developing and ageing rat brain. FEBS Lett. 85:301–304.

    Google Scholar 

  23. Giuffrida, A. M., Serra, I., Fiore, L., Vanella, A., andCozzolino, A. 1974. Biosynthesis of nucleic acids in different regions of rat brain. It. J. Biochem. 23:87–95.

    Google Scholar 

  24. Bondy, S. C., Roberts, S., andMorelos, B. S. 1970. Histone-acetylating enzyme of brain. Biochem. J. 119:665–672.

    Google Scholar 

  25. Gurley, L. R., D'Anna, J. A., Barham, S. S., Deaven, L. L., andTobey, R. A. 1978. Histone phosphorylation and chromatin structure during mitosis in Chinese Hamster cells. Eur. J. Biochem. 84:1–15.

    Google Scholar 

  26. Langan, T. A. 1969. Phosphorylation of liver histone following the administration of glucagon and insulin. Proc. Natl. Acad. Sci. USA 64:1276–1283.

    Google Scholar 

  27. Watson, G., andLangan, T. A. 1973. Effect of F1 histone and phosphorylated F1 histone on template activity of chromatin. Fed. Proc. 3:588 (abstract).

    Google Scholar 

  28. Rubin, C. S., andRosen, O. M. 1975. Protein phosphorylation. Ann. Rev. Biochem. 44:831–887.

    Google Scholar 

  29. De Lange, R. J., andSmith, E. L. 1971. Histones: structure and function. Ann. Rev. Biochem. 40:279–314.

    Google Scholar 

  30. Lee, C. T., andDuerre, J. A. 1974. Changes in histone methylase activity of rat brain and liver with ageing. Nature 251:240–242.

    Google Scholar 

  31. Duerre, J. A., andLee, C. T. 1974. In vivo methylation and turnover of rat brain histones. J. Neurochem. 23:541–547.

    Google Scholar 

  32. Fujitani, H., andHoloubek, V. 1974. Nonhistone nuclear proteins of rat brain. J. Neurochem. 23:1215–1224.

    Google Scholar 

  33. Heizmann, C. W., Arnold, E. M., andKuenzle, C. C. 1980. Fluctuations of non-histone chromosomal proteins in differentiating brain cortex and cerebellar neurons. J. Biol. Chem. 255:11504–11511.

    Google Scholar 

  34. Oh'Hara, I., andYanagihara, T. 1977. Nuclear chromatin proteins from rabbit cerebrum, cerebellum and liver: synthesis and phosphorylation. J. Neurochem. 29:1065–1073.

    Google Scholar 

  35. Yanagihara, T. 1980 Phosphorylation of chromatin proteins in cerebral anoxia and ischemia. J. Neurochem. 35:1209–1215.

    Google Scholar 

  36. Thakur, M. R., andKanungo, M. S. 1981. Methylation of chromosomal proteins and DNA of rat brain and its modulation by estradiol and calcium during ageing. Exp. Geront. 16:331–336.

    Google Scholar 

  37. Supakar, P. C., andKanungo, M. S. 1981. Chromatin function in developing brain: acetylation of chromosomal proteins and RNA synthesis. Biochem. Biophys. Res. Commun. 100:73–78.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biochemistry, University of Catania, Via'e Andrea Doria, 6, 95125, Catania, Italy

    I. Serra, M. Kamiyama, G. A. Hashim, P. Ragonese, B. Lombardo & A. M. Giuffrida

  2. St. Luke's Hospital Center, Columbia University, Via'e Andrea Doria, 6, 95125, New York, New York

    I. Serra, M. Kamiyama, G. A. Hashim, P. Ragonese, B. Lombardo & A. M. Giuffrida

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  1. I. Serra
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  2. M. Kamiyama
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  4. P. Ragonese
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  6. A. M. Giuffrida
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Serra, I., Kamiyama, M., Hashim, G.A. et al. Post-translational changes of chromosomal proteins in rat cerebellum during postnatal development. Neurochem Res 8, 1577–1587 (1983). https://doi.org/10.1007/BF00964159

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  • DOI: https://doi.org/10.1007/BF00964159

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