Neurochemical Research

, Volume 4, Issue 2, pp 277–287 | Cite as

Effect of administration of 5-hydroxytryptophan and an inhibitor ofl-aromatic amino acid decarboxylase on glucose metabolism in rat brain

  • Kar-Lit Wong
  • Gertrude M. Tyce
Original Articles


The effect of 5-hydroxytryptophan (5-HTP)—the precursor of serotonin (5-hydroxytryptamine, 5-HT)—and of an inhibitor,N-(dl-seryl)-N′-(2,3,4-trihydroxybenzyl)hydrazine (Ro4-4602), ofl-aromatic amino acid decarboxylase on the metabolism of glucose to amino acids in brain tissue was investigated. Labeled glucose (20 μCi, 0.24 mg in 0.2 ml 0.9% saline) was injected intravenously into fed rats pretreated with Ro4-4602 (50 mg/kg intraperitoneally) either alone or in combination with 5-HTP (30 mg/kg intravenously) or with the appropriate vehicle. After the injection of Ro4-4602 plus 5-HTP, the concentrations of 5-HT and 5-HTP in brain were increased, but the increase of 5-HTP that Ro4-4602 slightly inhibits the reaction of decarboxylation in the brain, although at the dose used the drug is usually considered to act only peripherally. After administration of Ro4-4602 alone or combined with 5-HTP, the concentration of glucose in plasma was not significantly increased. However, the concentration of glucose in brain was markedly increased with such treatments. The administration of Ro4-4602 alone or combined with 5-HTP reduced the flux of14C from labeled glucose to amino acids in brain. The concentrations of amino acids in brain were little changed by these treatments.


Glucose Brain Tissue Glucose Metabolism Hydrazine Acid Decarboxylase 
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Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • Kar-Lit Wong
    • 1
  • Gertrude M. Tyce
    • 1
  1. 1.Mayo Clinic and Mayo FoundationRochester

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