Abstract
[Me-3H] choline and [32P] orthophosphate were injected intraventricularly into adult female rats. After variable intervals from injection (1–10 min) the animals were sacrificed by means of a microwave apparatus, and phosphorylcholine and choline phosphoglycerides extracted from brain and counted for radioactivity content after separation. The kinetic constants (K) for phosphorylcholine incorporation into lipids were determined both for [32P] and [3H] labeling. From the data obtained by these procedures it is concluded that base-exchange reactions for choline incorporation into lipids are operating in rat brain in vivo and that they represent a rapidly equilibrating system.
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Orlando, P., Arienti, G., Cerrito, F. et al. Quantitative evaluation of two pathways for phosphatidylcholine biosynthesis in rat brain in vivo. Neurochem Res 2, 191–201 (1977). https://doi.org/10.1007/BF00964096
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DOI: https://doi.org/10.1007/BF00964096