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Immunochemical characterization of synaptosomal membrane antigens from chicken brain

Histochemical demonstration of changes in distribution during development

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Abstract

The developmental changes in the distribution of a number of nerve specific synaptic membrane antigens have been investigated in a number of tissues using indirect immunofluorescence histochemistry. In all tissues examined, by the time synaptic contacts were made, all regions of tissue that were going to display these antigens had already done so. Areas of tissue rich in actively dividing neuroblasts or postmitotic undifferentiated neurons showed little fluorescence. Characteristic strong fluorescence was only visible where regions of differentiated synapses or axons were present. In the optic nerve, and perhaps also in the white matter of the cerebellum and the outer plexiform layer of the retina, the antigens were already present before synapse formation. After synapse formation the antigens disappeared from the outer plexiform layer of the retina and the white matter of the cerebellum and spinal cord. The amount of fluorescence was drastically reduced in the deep cerebellar nuclei but did not change appreciably in the other areas examined. The loss of these antigens occurred at a different time in each of the tissue areas, indicating that it was related to the maturation of a particular network of neurons rather than the animal as a whole. By considering the pattern of developmental change of this set of antigens, the possibility that some of them may be involved in the processes of intercellular recognition or synaptogenesis has been examined.

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References

  1. Fox, T. O., Sheppard, J. R., andBurger, M. M. 1971. Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only in mitosis.Proc. Natl. Acad. Sci. US 68:244–247.

    Google Scholar 

  2. Bosmann, H. B. 1974. Cell plasma membrane external surface glycosyltransferases: activity in the cell mitotic cycle.Biochim. Biophys. Acta 339:438–441

    Google Scholar 

  3. Becker, F. F. 1974. Differential lectin agglutinability of fetal, dividing-postnatal and malignant hepatocytes.Proc. Natl. Acad. Sci. US 71:4307–4311.

    Google Scholar 

  4. Akeson, R., andHerschman, H. R. 1974. Modulation of cell-surface antigens of a murine neuroblastoma.Proc. Natl. Acad. Sci. US 71:187–191.

    Google Scholar 

  5. Brown, J. C. 1971. Surface glycoprotein characteristic of the differentiated state of neuroblastoma C-1300 cells.Exptl. Cell Research 69:440–442.

    Google Scholar 

  6. Truding, R., Shelanski, M. L., Daniels, M. P., andMorell, P. 1974. Comparison of surface membranes isolated from cultured murine neuroblastoma cells in the differentiated state.J. Biol. Chem. 249:3973–3982.

    Google Scholar 

  7. Joseph, B. S., andOldstone, M. B. A. 1974. Expression of selected antigens on the surface of cultured neural cells.Brain Research 80:421–434.

    Google Scholar 

  8. O'Lague, P. H., Obata, K., Claude, P., Furshpan, E. J., andPotter, D. D. 1974. Evidence for cholinergic synapses between dissociated rat sympathetic neurons in cell culture.Proc. Natl. Acad. Sci. US 71:3602–3606.

    Google Scholar 

  9. Livett, B. G., Rostas, J. A. P., Jeffrey, P. L., andAustin, L. 1974. Antigenicity of isolated synaptosomal membranes.Exptl. Neurol. 43:330–338.

    Google Scholar 

  10. Rostas, J. A. P., andJeffrey, P. L. 1977. Immunochemical characterization of synaptosomal membrane antigens from chicken brian. Histochemical localization in the day old chick.Neurochem. Research, 2:59–85.

    Google Scholar 

  11. Rostas, J. A. P., andJeffrey, P. L. 1977. Immunochemical characterization of synaptosomal membrane antigens from chicken brain. Quantitative determination and partial characterization. Neurochem. Research, in press.

  12. Hamburger, V., andHamilton, H. L. 1951. A series of normal stages in the development of the chick embryo.J. Morphol 88:49–92.

    Google Scholar 

  13. Foelix, R. F., andOppenheim, R. 1974. The development of synapses in the cerebellar cortex of the chick embryo.J. Neurocytol. 3:277–294.

    Google Scholar 

  14. Fonnum, F., andWalberg, F. 1973. An estimation of the concentration of γ-aminobutyric acid and glutamate decarboxylase in the inhibitory Purkinje cell terminals in the cat.Brain Research 54:115–127.

    Google Scholar 

  15. McGeer, P. L., Hattori, T., andMcGeer, E. G. 1975. Chemical and autoradiographic analysis of γ-aminobutyric acid transport in Purkinje cells of the cerebellum.Exptl. Neurol. 47:26–41.

    Google Scholar 

  16. Ramon y Cajal, S. 1960. Studies on vertebrate neurogenesis,Guth, L. (trans.). Charles C. Thomas, Springfield, Ill.

    Google Scholar 

  17. Coulombre, A. J. 1955. Correlations of structural and biochemical changes in the developing retina of the chick.Am. J. Anat. 96:153–189.

    Google Scholar 

  18. Shen, S.-C., Greenfield, P., andBoell, E. J. 1956. Localization of acetylcholinesterase in chick retina during histogenesis.J. Comp. Neurol. 106:433–461.

    Google Scholar 

  19. Hartman, B. K., Zide, D., andUdenfriend, S. 1972. The use of dopamine β-hydroxylase as a marker for the central noradrenergic nervous system in the rat.Proc. Natl. Acad. Sci. US 69:2722–2726.

    Google Scholar 

  20. Margolis, R. K., andGomez, Z. 1974. Structural changes in brain glycoproteins during development.Brain Research 74:370–372.

    Google Scholar 

  21. Rostas, J. A. P., andJeffrey, P. L. 1975. Restricted mobility of neuronal membrane antigens.Neurosci. Lett. 1:47–53.

    Google Scholar 

  22. Landis, D. M. D., andReese, T. S. 1974. Differences in membrane structure between excitatory and inhibitory synapses in the cerebellar cortex.J. Comp. Neurol. 155:93–126.

    Google Scholar 

  23. Landis, D. M. D., Reese, T. S., andRaviola, E. 1974. Differences in membrane structure between excitatory and inhibitory components of the reciprocal synapse in the olfactory bulb.J. Comp. Neurol. 155:67–92.

    Google Scholar 

  24. Balazs, R., Hajos, F., Johnson, A. L., Reynierse, G. L. A., Tapia, R., andWilkin, G. P. 1975. Subcellular fractionation of rat cerebellum: an electron microscopic and biochemical investigation. III. Isolation of large fragments of the cerebellar glomeruli.Brain Research 86:17–30.

    Google Scholar 

  25. Hajos, F., Tapia, R., Wilkin, G., Johnson, A. L., andBalazs, R. 1974. Subcellular fractionation of rat cerebellum: an electron microscopic and biochemical investigation. I. Preservation of large fragments of the cerebellar glomeruli.Brain Research 70:261–279.

    Google Scholar 

  26. Hajos, F., Wilkin, G., Wilson, J., andBalazs, R. 1975. A rapid procedure for obtaining a preparation of large fragments of the cerebellar glomeruli in high purity.J. Neurochem. 24:1277–1278.

    Google Scholar 

  27. Tapia, R., Hajos, F., Wilkin, G., Johnson, A. L., andBalazs, R. 1974. Subcellular fractionation of rat cerebellum: an electron microscopic and biochemical investigation. II. Resolution of morphologically characterized fractions.Brain Research 70:285–299.

    Google Scholar 

  28. Marshall, J., Medford, P. A., andVoaden, M. J. 1974. Subcellular fractionation of the rabbit retina: the isolation of synaptic pedicles and inner segments of photoreceptor cells.Exptl. Eye Research 19:559–569.

    Google Scholar 

  29. Neal, M. J., andAtterwill, C. K. 1974. Isolation of photoreceptor and conventional nerve terminals by subcellular fractionation of rabbit retina.Nature 251:331–333.

    Google Scholar 

  30. Wilson, W. S., andCooper, J. R. 1972. The preparation of cholinergic synaptosomes from bovine superior cervical ganglia.J. Neurochem. 19:2779–2790.

    Google Scholar 

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Author notes

  1. John A. P. Rostas

    Present address: Department of Psychobiology, University of California, 92717, Irvine, California

Authors and Affiliations

  1. Department of Biochemistry, Monash University, 3168, Clayton, Victoria, Australia

    John A. P. Rostas & Peter L. Jeffrey

Authors
  1. John A. P. Rostas
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  2. Peter L. Jeffrey
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Rostas, J.A.P., Jeffrey, P.L. Immunochemical characterization of synaptosomal membrane antigens from chicken brain. Neurochem Res 2, 171–189 (1977). https://doi.org/10.1007/BF00964095

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  • DOI: https://doi.org/10.1007/BF00964095

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