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Neurochemical Research

, Volume 8, Issue 2, pp 193–206 | Cite as

Cytotoxic effects of methylnitrosourea on developing brain

  • Kannosuke Fujimori
  • Momoko Sunouchi
  • Kazuhide Inoue
  • Masahiro Nakadate
  • Akira Takanaka
  • Yoshihito Omori
Original Articles

Abstract

The effect of methylnitrosourea (MNU) on cerebellar and cerebral DNA, RNA, protein, lysosomal enzymes (acid DNase, RNase, phosphatase, and beta-glucuronidase), and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (2′,3′-CNPase) activities was studied in rats from birth through 12 days of age. Subcutaneous injection of MNU in a dose of 0.625 mmol/kg caused a suppression of increase in weights and content of DNA, RNA, and protein of cerebellum, but no changes in those of the cerebrum or in body weight. Ratios of protein and RNA to DNA were substantially elevated by MNU in the cerebellum but not in the cerebrum.

Acid DNase and acid RNase activities of MNU-treated rats were significantly elevated beyond the increase of these activities in controls in the cerebellum, but no change in these activities by MNU was observed in the cerebrum. A slight elevation in acid phosphatase activity was observed in the cerebellum but not in the cerebrum after MNU pretreatment. Beta-glucuronidase and 2′,3′-CNPase activities were not changed in the cerebellum or in the cerebrum. These results suggest that in the developing brain, especially in the cerebellum at the mitotic stage, MNU caused cell damage and inhibited cell mitosis.

Keywords

Cytotoxic Effect Phosphatase Activity Cell Damage Acid Phosphatase Subcutaneous Injection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Kannosuke Fujimori
    • 1
  • Momoko Sunouchi
    • 1
  • Kazuhide Inoue
    • 1
  • Masahiro Nakadate
    • 2
  • Akira Takanaka
    • 1
  • Yoshihito Omori
    • 1
  1. 1.Division of Pharmacology Biological Safety Research CenterNational Institute of Hygienic SciencesTokyoJapan
  2. 2.Department of Synthetic ChemistryNational Institute of Hygienic SciencesTokyoJapan

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