Neurochemical Research

, Volume 6, Issue 6, pp 699–718 | Cite as

Successful immunization against experimental allergic encephalomyelitis with myelin basic protein-sensitized allogeneic lymphocytes

  • George A. Hashim
Original Articles

Abstract

Prevention and suppression of experimental allergic encephalomyelitis were demonstrated in rats, guinea pigs, and rabbits immunized with allogeneic, but not with syngeneic lymphocytes from susceptible donors sensitized to myelin basic protein (MBP). Donor lymphnode, splenic, or peripheral blood lymphocytes were effective in inducing a state of unresponsiveness to an encephalitogenic challenge in either of the three species. Unresponsiveness was not obtained in recipients immunized with sensitized allogenic lymphocytes and simulatenously challenged with MBP suggesting that a time lapse between immunization and challenge is necessary for the development of protective immunity. Induced in immunized recipients, unresponsiveness was transferred into normal syngeneic recipients with immunoglobulin-G (IgG) isolated from protected donors before challenge. Furthermore, both immunized and IgG recipients failed to develop cell-mediated immunity after challenge with MBP. The results show that prevention and suppression of EAE was mediated by antibodies which inhibited the development of delayed type hypersensitivity to the challenging antigen.

Keywords

Peripheral Blood Lymphocyte Myelin Basic Protein Encephalomyelitis Protective Immunity Time Lapse 

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Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • George A. Hashim
    • 1
  1. 1.The Jane Forbes Clark Surgical Research Laboratories Department of Surgery and MicrobiologySt. Luke's-Roosevelt Hospital Center and Columbia UniversityNew York

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