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Neurochemical Research

, Volume 4, Issue 1, pp 63–72 | Cite as

Gangliosides and other lipid micelles. A study of amine binding by a dialysis/fluorescence method

  • Howard C. Price
  • Christopher Byard
  • William Sims
  • Raymond Wilson
Original Articles

Abstract

The binding of serotonin to bovine adrenal medulla gangliosides (BAMG), bovine brain gangliosides (BBG), and BBG-synthetic lecithin mixed micelles has been demonstrated by a rapid technique using fluorescence monitoring of dialysis rates. BAMG micelles bound 0.10 mM serotonin at about half the efficiency of BBG micelles having the same sialic acid concentration (0.50 mM) in water. In water, the effect of BBG micelles was essentially identical to that of mixed micelles containing the same quantity of BBG. Ca2+ (1.20 mM), however, cancelled the effect of the mixed micelles on serotonin dialysis and reduced the effect of BBG micelles to about one quarter of that observed in water. These and other observations are related to earlier studies and to neurochemical processes which may involve gangliosides.

Keywords

Lipid Serotonin Acid Concentration Lecithin Sialic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Wiegandt, H. 1967. The subcellular localization of gangliosides in the brain. J. Neurochem. 14:671–674.Google Scholar
  2. 2.
    Wooley, D. W., andGommi, B. W. 1965. Serotonin receptors, VII. activities of various pure gangliosides as the receptors. Proc. Natl. Acad. Sci. U.S.A. 53:959–963.Google Scholar
  3. 3.
    Wiegandt, H. 1968. The structure and the function of gangliosides. Angew. Chem. Int. Ed. (Engl.) 7:87–96.Google Scholar
  4. 4.
    Van Heyningen, W. E. 1974. Gangliosides as membrane receptors for tetanus toxin, cholera toxin, and serotonin. Nature 249:415–417.Google Scholar
  5. 5.
    Simpson, L. L., andRapport, M. M. 1971. The binding of botulinum toxin to membrane lipids: Sphingolipids, steroids, and fatty acids. J. Neurochem. 18:1751–1759.Google Scholar
  6. 6.
    Gielen, W. 1966. The function of gangliosides. A serotonin and calcium receptor. Z. Naturforsch. 21:1007–1008.Google Scholar
  7. 7.
    Van Heyningen, W. E. 1963. The fixation of tetanus toxin, strychnine, serotonin, and other substances by ganglioside. J. Gen. Microbiol. 31:375–387.Google Scholar
  8. 8.
    Fishman, P. H. andBrady, R. O. 1976. Biosynthesis and function of gangliosides. Science 194:906–915.Google Scholar
  9. 9.
    Ochoa, E. L. M., andBangham, A. D. 1976.N-Acetylneuraminic acid molecules as possible serotonin binding sites. J. Neurochem. 26:1193–1198.Google Scholar
  10. 10.
    Cuatrecasus, P., Parikh, I., andHollenberg, M. D. 1973. Affinity chromatography and structural anslysis ofVibrio cholerae enterotoxin-ganglioside agarose and the biological effects of ganglioside-containing soluble polymers. Biochemistry 12:4253–4264.Google Scholar
  11. 11.
    Albers, R. W., andKoval, G. J. 1962. The interaction of gangliosides with cationic molecules. Biochim. Biophys. Acta 60:359–365.Google Scholar
  12. 12.
    Abramson, M. B., Yu, R. K., andZaby, V. 1972. Ionic properties of beef brain gangliosides. Biochim. Biophys. Acta 280:365–372.Google Scholar
  13. 13.
    Byard, C., Lockney, M., andPrice, H. C. 1975. The interaction of gangliosides with putative neurotransmitters. A chromatographic study. 169th National Meeting of The American Chemical Society, Philadelphia, Pa. April 6–11.Google Scholar
  14. 14.
    Ledeen, R. W., Yu, R. K., andEng, L. F. 1973. Gangliosides of human myelin: Sialosylgalactosylceramide (G7) as a major component. J. Neurochem. 21:829–839.Google Scholar
  15. 15.
    Price, H. C., andYu, R. K. 1976. Adrenal medulla gangliosides. A comparative study of some mamals. Comp. Biochem. Physiol. 54B:451–454.Google Scholar
  16. 16.
    Yu, R. K., andLudeen, R. W. 1970. Gas-liquid chromatographic assay of lipid-bound sialic acids: measurement of gangliosides in brain of several species. J. Lipid Res. 11:506–516.Google Scholar
  17. 17.
    Jourdian, G. W., Dean, L., andRoseman, S. 1971. The sialic acids. XI. a periodate resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J. Biol. Chem. 246:430–435.Google Scholar
  18. 18.
    Suzuki, K. 1964. A simple and accurate micromethod for quantitative determination of ganglioside patterns. Life Sci. 3:1227–1233.Google Scholar
  19. 19.
    Ledeen, R., Salsman, K., andCabrera, M. 1968. Gangliosides of bovine adrenal medulla. Biochemistry 7:2287–2295.Google Scholar
  20. 20.
    Gammack, D. B. 1963. Physiochemical properties of ox-brain gangliosides. Biochem. J. 88:373–383.Google Scholar
  21. 21.
    Veinberg, A. Y., Chupriyanova, N. E., Rodionov, Y. V., andSamokhvalov, G. I. 1975. Certain features of the interaction of gangliosides with serotonin. Biofizika 20:388–393.Google Scholar
  22. 22.
    Price, H., Kundu, S., andLedeen, R. 1975. Structures of gangliosides from bovine adrenal medulla. Biochemistry 14:1512–1518.Google Scholar
  23. 23.
    Puro, K. 1970. Isolation of bovine kidney gangliosides. Acta Chem. Scand. 24:13–22.Google Scholar

Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • Howard C. Price
    • 1
  • Christopher Byard
    • 1
  • William Sims
    • 1
  • Raymond Wilson
    • 1
  1. 1.Department of ChemistryMarshall UniversityHuntington

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