Abstract
Separation and quantitation of polyamines from unpollinated pea (Pisum sativum L.) ovaries and young fruits induced by application of gibberellic acid to unpollinated ovaries showed, in both cases, a decrease in putrescine and spermidine levels between anthesis and 4 d later. By contrast, spermine levels increased prior to the onset of senescence of the unpollinated ovaries (3 d post anthesis) and decreased during fruit development. Low levels of putrescine, spermidine and spermine were also observed in young fruits obtained by self-pollination and by treatment of unpollinated ovaries with 2,4-dichlorophenoxyacetic acid. In-vitro culture of ovary explants in a medium containing spermine showed that a reduction of the growth of gibberellic acid-treated unpollinated ovaries was associated with a rise in the level of spermine in the fruits. The results obtained indicate that changes in spermine levels are involved in the control of ovary senescence and of fruit set and development.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophen-oxyacetic acid
- GA3 :
-
gibberellic acid
- HPLC:
-
high-performance liquid chromatography
References
Biasi, R., Bagni, N., Costa, G. (1988) Endogenous polyamines in apple and their relationship to fruit set and fruit growth. Physiol. Plant73, 201–205
Carbonell, J., García-Martínez, J.L. (1980) Fruit-set of unpollinated ovaries ofPisum sativum L. Influence of vegetative parts. Planta147, 444–450
Carbonell, J., García-Martínez, J.L. (1985) Ribulose-1,5-bisphosphate carboxylase and fruit set or degeneration of unpollinated ovaries ofPisum sativum. Planta164, 534–539
Carrasco, P., Carbonell, J. (1988) Involvement of a neutral proteolytic activity in the senescence of unpollinated ovaries ofPisum sativum L. Physiol. Plant72, 610–616
Dai, Y.-R., Kaur-Sawhney, R., Galston, A.W. (1982) Promotion by gibberellic acid of polyamine biosynthesis in inter-nodes of light-grown dwarf peas. Plant Physiol.69, 103–105
Downs, C.G., Lovell, P.H. (1986) The effect of spermidine and putrescine on the senescence of cut carnations. Physiol. Plant66, 679–684
Federico, R., Angelini, R. (1988) Distribution of polyamines and their related catabolic enzyme in etiolated and light-grown leguminosae seedlings. Planta173, 317–321
Flores, H.E., Galston, A.W. (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol69, 701–706
Galston, A.W., K-Sawhney, R. (1987) Polyamines and senescence in plants. In: Plant senescence: Its biochemistry and physiology, pp. 167–181, Thomson, W.W., Nothnagel, E.A., Huffaker, R.C., eds. The American Society of Plant Physiologists
García-Martínez, J.L., Carbonell, J. (1980) Fruit-set of unpollinated ovaries ofPisum sativum L. Influence of plant growth regulators. Planta147, 451–456
García-Martínez, J.L., Carbonell, J. (1985) Induction of fruitset in pea ovary explants by gibberellic acid. J. Plant Growth Regul.4, 19–27
García-Martínez, J.L., Sponsel, V.M., Gaskin, P. (1987) Gibberellins in developing fruits ofPisum sativum cv. Alaska: Studies on their role in pod growth and seed development. Planta170, 130–137
Goren, R., Palavan, N., Flores, H.E., Galston, A.W. (1982) Changes in polyamine titer in etiolated pea seedlings following red light treatment. Plant Cell Physiol.23, 19–26
Icekson, I., Bakhanashvili, M., Apelvaum, A. (1986) Inhibition by ethylene of polyamine biosynthetic enzymes enhanced lysine decarboxylase activity and cadaverine accumulation in pea seedlings. Plant Physiol.82, 607–609
Kaur-Sawhney, R., Galston, A.W. (1979) Interaction of polyamines and light on biochemical processes involved in leaf senescence. Plant Cell Environ.2, 189–196
Peretó, J.G., Beltrán, J.P., García-Martínez, J.L. (1988) The source of gibberellins in the parthenocarpic development of ovaries on topped pea plants. Planta175, 493–499
Slocum, R.D., Galston, A.W. (1985) Changes in polyamine biosynthesis associated with postfertilization growth and development in tobacco ovary tissues. Plant Physiol.79, 336–343
Smith, T.A. (1985) Polyamines. Annu. Rev. Plant Physiol.36, 117–143
Smith, M.A. Davies, P.J. (1985) Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives. Plant Physiol.78, 89–91
Smith, M.A., Davies, P.J., Reid, J.B. (1985) Role of polyamines in gibberellin-induced internode growth in peas. Plant Physiol.78, 92–99
Vercher, Y., Molowny, A., Carbonell, J. (1987) Gibberellic acid effects on the ultrastructure of endocarp cells of unpollinated ovaries ofPisum sativum. Physiol. Plant71, 302–308
Vercher, Y., Molowny, A., López, C., García-Martínez, J.L., Carbonell, J. (1984) Structural changes in the ovary ofPisum sativum L. Induced by pollination and gibberellic acid. Plant Sci. Lett.36, 87–91
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Carbonell, J., Navarro, J.L. Correlation of spermine levels with ovary senescence and with fruit set and development inPisum sativum L.. Planta 178, 482–487 (1989). https://doi.org/10.1007/BF00963818
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DOI: https://doi.org/10.1007/BF00963818