Planta

, Volume 178, Issue 4, pp 475–481 | Cite as

Short-term carbon-isotope discrimination in C3−C4 intermediate species

  • Susanne von Caemmerer
  • Kerry T. Hubick
Article

Abstract

Short-term discrimination in assimilation of stable isotopes of carbon was measured for leaves of the C3 speciesPhaseolus vulgaris L. cv. Hawkesbury Wonder andFlaveria pringlei Gandoger, the C4 speciesAmaranthus edulis Speg., and the C3−C4 intermediate speciesPanicum milioides Nees ex. Trin,Flaveria floridana Johnson, andFlaveria anomala B.L. Robinson. Discriminations in the C3 and C4 species were similar to those expected from theoretical considerations. When ambient CO2 pressure was 330 μbar the mean discriminations in the C3 species andPanicum milioides were similar, whereas the mean discriminations inF. floridana andF. anomala were less than discrimination in C3 species andPanicum milioides. When ambient CO2 pressure was 100 μbar the mean discriminations inPanicum milioides andF. anomala were greater, and that inF. floridana was less, than that inPhaseolus vulgaris. We conclude that the pattern of discrimination inPanicum milioides is consistent with the presence of a glycine shuttle; inF. floridana andF. anomala, discrimination is consistent with the presence of a C4 pathway coupled with the operation of a glycine shuttle.

Key words

C3−C4 intermediates Carbon isotope discrimination Flaveria Panicum Photosynthesis (C3−C4

Abbreviations and symbols

PEP

phosphoenolpyruvate

Rubisco

ribulose, 1,5-bisphosphate carboxylase-oxygenase (EC 4.1.1.39)

pa

ambient CO2 pressure

pi

intercellular CO2 pressure

Δ

carbon-isotope discrimination

δ

carbonisotope composition relative to PeeDee Belemnite

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Susanne von Caemmerer
    • 1
  • Kerry T. Hubick
    • 1
  1. 1.Plant Environmental Biology Group, Research School of Biological SciencesAustralian National UniversityCanberra CityAustralia

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