Planta

, Volume 178, Issue 4, pp 425–435 | Cite as

A new organelle related to osmoregulation in ultrarapidly frozenPelvetia embryos

  • John C. Gilkey
  • L. Andrew Staehelin
Article

Abstract

Freeze-fracture electron microscopy of the cortical cytoplasm of unfixed, uncryoprotected, ultrarapidly frozen embryos of the marine brown algaPelvetia fastigiata has demonstrated the presence of numerous 0.5-μm diameter, disc-shaped vesicles lying adjacent and nearly parallel to the plasma membrane. Some vesicles are fused with the plasma membrane through a narrow connection; this however appears to be a reversible attachment rather than an intermediate stage in the incorporation of the vesicle into the plasma membrane. The distribution of these connections in the plane of the membrane is not uniform; they tend to occur in patches. The fraction of vesicles that is fused with the plasma membrane at any one time appears to be related to a cell's perception of a stressful hypotonic imbalance between the internal and external concentrations of osmotically active compounds. Thus, a sudden 5% decrease in osmolarity of the artificial seawater medium just before freezing leads to a 38% increase in connections per unit membrane area, while a 20% decrease in osmolarity leads to a 75% increase in connections per unit area. Based on these findings and the corresponding ion-transport studies of R. Nuccitelli and L.F. Jaffe (1976, Planta131, 315–320), we postulate that the disc-shaped vesicles mediate short-term osmoregulation inPelvetia embryos by reversibly inserting chloride channels into the plasma membrane.

Key words

Ion transport Membrane fusion Osmoregulation Plasma membrane Pelvetia Phaeophyta 

Abbreviations

ASW

artificial sea water

IMP

intramembrane particle

EF

fracture face of a freeze-fractured exoplasmic membrane leaflet

PF

fracture face of a protoplasmic membrane leaflet

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • John C. Gilkey
    • 1
  • L. Andrew Staehelin
    • 1
  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of ColoradoBoulderUSA

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