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Neurochemical Research

, Volume 4, Issue 3, pp 339–354 | Cite as

High-affinity uptake of γ-aminobutyric acid in cultured glial and neuronal cells

  • V. J. Balcar
  • J. Mark
  • J. Borg
  • P. Mandel
Original Articles

Abstract

Both glial and neuronal cells maintained in primary culture were found to accumulate [3H]GABA by an efficient “high-affinity” uptake system (apparentKm=9 μM,Vmax=0.018 and 0.584 nmol/mg/min, respectively) which required sodium ions and was inhibited by 1 mM ouabain. Strychnine and parachloromercuriphenylsulfonate (pCS) (both at 1 mM) also strongly inhibited uptake of [3H]GABA, but metabolic inhibitors (2,4-dinitrophenol, potassium cyanide, and malonate) were without effect. Only three structural analogs of GABA (nipecotate, β-alanine, and 2,4-diaminobutyrate) inhibited uptake of [3H]GABA, while several other compounds with structural similarities to GABA (e.g. glycine,l-proline, and taurine) did not interact with the system. The kinetic studies indicated presence of a second uptake (Km=92 μM,Vmax=0.124 nmol/mg/min) in the primary cultures containing predominantly glioblasts. On the other hand, only one of the neuronal cell lines transformed by simian virus SV40 appeared to accumulate [3H]GABA against a concentration gradient. ApparentKm of this uptake was relatively high (819 μM), and it was only weakly inhibited by 1 mM ouabain and 1 mM pCS. The structural specificity also differed from that of the uptake observed in the primary cultures. Significantly, none of the nontransformed continuous cell lines of either tumoral (glioma, C6; neuroblastoma, Ml; MINN) or normal (NN; I6) origin actively accumulated [3H]GABA. It is suggested that for the neurochemical studies related to GABA and requiring homogeneous cell populations, the primary cultures offer a better experimental model than the continuous cell lines.

Keywords

Neuronal Cell Neuroblastoma Taurine Primary Culture Ouabain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • V. J. Balcar
    • 1
  • J. Mark
    • 1
  • J. Borg
    • 1
  • P. Mandel
    • 1
  1. 1.Centre de Neurochimie du CNRSInstitut de Chimie BiologiqueStrasbourg CedexFrance

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