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Neurochemical Research

, Volume 3, Issue 5, pp 563–571 | Cite as

Distribution and release of adenosine triphosphate in rat brain

  • P. H. Wu
  • J. W. Phillis
Original Articles

Abstract

The steady-state level of brain ATP was measured after the tissue had been treated with a focused microwave irradiation system. The brain ATP content (1.50 nmol/mg tissue) obtained by microwave fixation is similar to that observed by others using fast-freezing and microwave fixation techniques. The concentrations of ATP in different brain regions show a rather uniform distribution, ranging from 1.918±0.059 (brainstem) to 2.393±0.19 (caudate) nmol/mg tissue; however, insufficient microwave fixation time seems to produce a greater regional variation of ATP. Release of ATP was investigated by placing a cup on the sensory-motor cortex. The rate for basal release of ATP is 1.43±0.14 femtomole/min/mm2. A 30-fold increase in ATP release was obtained by direct stimulation of the cortex with 5 mA pulses of 0.2 msec duration at a rate of 20/sec over a period of 10 min. These results, in conjunction with others describing the potent pharmacological action of the nucleotide, seem to suggest that ATP could be a mediator in a “purinergic” system in the CNS.

Keywords

Adenosine Triphosphate Microwave Irradiation Fixation Time Pharmacological Action 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • P. H. Wu
    • 1
  • J. W. Phillis
    • 1
  1. 1.Department of Physiology College of MedicineUniversity of SaskatchewanSaskatoonCanada

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