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Electron microscopic studies on the effects of amanitin in mice: liver and heart lesions

  • J. Meldolesi
  • G. Pelosi
  • A. Brunelli
  • E. Genovese
Article

Summary

In the liver, lesions of the hepatocytes, Kupffer and endothelial cells are present. The hepatocytes show alterations of sinusoidal microvilli, large vacuoles delimited by a membrane continuous with the plasma membrane, and interruptions of the plasma membrane.

In the skeletal muscle only rare and mild alterations are observed. In the heart the early lesions seem to be located in the capillaries, which present swelling of endothelial cells and absence of pinocytotic vesicles. Successively severe lesions (consisting of endocellular edema and rupture of the plasma membrane with extrusion of the cellular organelles) also appear in the myocardial cells.

It may be deduced that amanitin probably exerts a noxious action at the level of the plasma membrane.

Keywords

Public Health Endothelial Cell Plasma Membrane Skeletal Muscle Microscopic Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

ktronenmikroskopische untersuchungen über die wirkung von amanitin auf die maus: Veränderungen in leber und herz

Zusammenfassung

Die Leberzellen zeigen Veränderungen der sinusoidalen Mikrovilli, große Vacuolen, die von einer mit der Plasmamembran kontinuierlich zusammenhängenden Membran begrenzt sind und Unterbrechungen der Plasmamembran. Im Skeletmuskel sind selten leichte Veränderungen zu sehen. Die Frühveränderungen im Herzen scheinen in den Capillaren lokalisiert zu sein, welche Schwellung der Endothelzellen und Fehlen von Pinocytosebläschen zeigen. Schritt für Schritt treten dann auch schwere Veränderungen in den Myokardzellen auf, wie endocelluläres Ödem, Ruptur der Plasmamembran mit Austritt von Zellorganellen. Man kann daraus schließen, daß das Amanitin wahrscheinlich seine Giftwirkung an der Plasmamembran entfaltet.

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Copyright information

© Springer-Verlag 1967

Authors and Affiliations

  • J. Meldolesi
    • 1
    • 2
  • G. Pelosi
    • 1
    • 2
  • A. Brunelli
    • 1
    • 2
  • E. Genovese
    • 1
    • 2
  1. 1.Institute of Pharmacology of the University of MilanItaly
  2. 2.Section for the Investigation of Subcellular Structures and Laboratory of ToxicologyItaly

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